尼古丁对离体大鼠心脏功能的抑制作用。

Nicotine-depressed function in isolated rat heart.

作者信息

McGrath J J, Smith D

出版信息

Drug Chem Toxicol. 1984;7(1):1-10. doi: 10.3109/01480548409014169.

Abstract

Experiments were conducted to assess the effects of nicotine on the isolated rat heart. Hearts removed from laboratory rats were perfused via the aorta with oxygenated (95% O2-5% CO2) Krebs-Henseleit solution. After 30 min, the hearts were challenged with perfusate containing nicotine. Heart rate and coronary flow decreased in response to nicotine concentrations of 5 micrograms/ml or above. Solutions containing 5, 10 and 20 micrograms/ml nicotine depressed heart rate by 9, 29 and 25%, and coronary flow by 11, 28 and 33%. Pulse pressure was significantly depressed by 20 micrograms/ml nicotine. Perfusion with nicotine-free solution for 10 min completely reversed the decreases in heart rate, coronary flow and pulse pressure. These results indicate that nicotine continuously infused into the isolated rat heart depresses heart rate and coronary flow in a dose-response manner and that these changes are reversible over the range of concentrations used.

摘要

进行实验以评估尼古丁对离体大鼠心脏的影响。从实验大鼠身上取出的心脏通过主动脉用含氧（95% O₂ - 5% CO₂）的克雷布斯 - 亨泽莱特溶液进行灌注。30分钟后，用含尼古丁的灌注液对心脏进行刺激。当尼古丁浓度达到5微克/毫升或更高时，心率和冠状动脉血流量会下降。含有5、10和20微克/毫升尼古丁的溶液使心率分别降低了9%、29%和25%，冠状动脉血流量分别降低了11%、28%和33%。20微克/毫升的尼古丁使脉压显著降低。用无尼古丁溶液灌注10分钟可完全逆转心率、冠状动脉血流量和脉压的下降。这些结果表明，持续注入离体大鼠心脏的尼古丁以剂量反应方式降低心率和冠状动脉血流量，并且在所用浓度范围内这些变化是可逆的。