a Department of Biological Engineering, College of Engineering , Konkuk University , Seoul , Republic of Korea.
b Daisung Green Tech , Gyeonggi-do , Republic of Korea.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):621-625. doi: 10.1080/21691401.2019.1574264.
A new method has been developed for the simple, fluorescence, turn-on detection of melamine, which utilizes DNA-templated silver nanoclusters (DNA-AgNCs) as a key component. In the sensor, melamine exhibits the dual functions: one is to enhance the fluorescence signal of DNA-AgNCs by its specific interaction with thymine residues in DNA template, and the other is to prevent Hg(II)-induced fluorescence quenching of DNA-AgNCs via its strong coordination with Hg(II). These consequently enable the sensitive and selective detection of melamine. By exploiting such novel features of melamine, we significantly increased the fluorescence response up to 360%, compared to the previous counterpart that relies on DNA-AgNCs only, and successfully determined melamine down to ca. 49 nM, a value that is 400 times lower than the safety level of 20 μM set by the US Food and Drug Administration. In addition, it was confirmed that the proposed approach works fine even in the real milk samples without any additional pre-treatment steps.
一种新方法已被开发用于简单、荧光、开启检测三聚氰胺，该方法利用 DNA 模板银纳米簇（DNA-AgNCs）作为关键组件。在传感器中，三聚氰胺通过与 DNA 模板中的胸腺嘧啶残基的特异性相互作用表现出双重功能：一是增强 DNA-AgNCs 的荧光信号，二是通过与 Hg(II)的强配位防止 Hg(II)诱导的 DNA-AgNCs 的荧光猝灭。这些作用使得三聚氰胺的灵敏和选择性检测成为可能。通过利用三聚氰胺的这些新颖特性，与仅依赖 DNA-AgNCs 的先前对应物相比，我们将荧光响应显著提高了 360%，并成功地将三聚氰胺检测到低至约 49 nM，这一值比美国食品和药物管理局设定的 20 μM 的安全水平低 400 倍。此外，还证实即使在没有任何额外预处理步骤的实际牛奶样品中，该方法也能很好地工作。
