a Department of Biochemistry , Lorestan University of Medical Science , Khorramabad , Iran.
b Student Research Committee , Lorestan University of Medical Sciences , Khorramabad , Iran.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):891-895. doi: 10.1080/21691401.2019.1580286.
Astaxanthin, a Xanthophyll carotenoid, has strong antioxidant properties. Some studies have shown the effectiveness of this compound on the prevention and treatment of cancer. Therefore, the aim of this study was to evaluate the effects of astaxanthin on induction of apoptosis and antioxidant activity in the LS-180 cell line. In this experimental study, after the treatment of LS-180 50, 100 and 150 μm of Astaxanthin for 24 h, the expression levels of Bax, Bcl2 and Caspase3 genes were investigated by Real-time PCR. Also, the level of malondialdehyde, as an indicator of oxidative stress and activity of anti-superoxide dismutase enzymes, catalase and glutathione peroxidase was investigated by colorimetric methods. The results showed that astaxanthin increases the expression of Bax and Caspase3 genes and decreases that of Bcl2, thereby, inducing apoptosis and inhibiting growth and proliferation of the cells. Additionally, reduction in the levels of malondialdehyde was evident with a significant elevation in antioxidant activity mediated by the action of superoxide dismutase, catalase and glutathione peroxidase. These results suggest that astaxanthin has the potency to induce apoptosis in LS-180 cells by increasing the expression of apoptotic genes and activity of antioxidant enzymes. Thus, astaxanthin has potential in the prevention and treatment of cancer.
虾青素，一种叶黄素类胡萝卜素，具有很强的抗氧化特性。一些研究表明，这种化合物在预防和治疗癌症方面具有有效性。因此，本研究旨在评估虾青素对 LS-180 细胞系细胞凋亡诱导和抗氧化活性的影响。在这项实验研究中，用虾青素处理 LS-180 50、100 和 150μm 24 小时后，通过实时 PCR 检测 Bax、Bcl2 和 Caspase3 基因的表达水平。此外，通过比色法检测丙二醛（氧化应激的指标）以及超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。结果表明，虾青素增加了 Bax 和 Caspase3 基因的表达，降低了 Bcl2 的表达，从而诱导细胞凋亡并抑制细胞的生长和增殖。此外，丙二醛水平的降低伴随着超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶介导的抗氧化活性的显著升高。这些结果表明，虾青素通过增加凋亡基因的表达和抗氧化酶的活性，具有诱导 LS-180 细胞凋亡的潜力。因此，虾青素在癌症的预防和治疗方面具有潜力。
