Nawroth P P, Stern D M, Kisiel W, Dietrich M
Br J Haematol. 1986 Jun;63(2):309-20. doi: 10.1111/j.1365-2141.1986.tb05554.x.
In this report cultured bovine capillary endothelial cells are demonstrated to specifically bind factors IX and X and also their activated forms. Bound factor IXa and cell-associated factor VIII can activate factor X. The product of this reaction, factor Xa, can then interact with a factor V-like molecule expressed by capillary endothelial cells promoting thrombin formation. The thrombin formed can cleave fibrinogen leading to release of fibrinopeptide A and clot formation. Endotoxin-treatment of capillary endothelial cells leads to induction of tissue factor activity which, in the presence of factor VIIa, promotes activation of factors IX and X. The amount of factor Xa formed endotoxin-treated endothelial cells incubated with factors VIIa, IX, VIII and X, is 8 times greater than cells incubated with factors VIIa and X alone. This indicates that on the perturbed endothelial cell surface factors VIII and IX do play an important role in factor X activation by the tissue factor pathway. The perturbed capillary endothelial cell can thus provide a model of the thrombotic state promoting initiation and propagation of a procoagulant pathway leading to thrombin formation. This pathway of coagulation is endothelial cell-dependent, since it requires expression of tissue factor and factor V by capillary endothelial cells, as well as interaction of coagulation factors with the surface of capillary endothelial cells.
在本报告中，已证明培养的牛毛细血管内皮细胞可特异性结合因子IX和X及其活化形式。结合的因子IXa和细胞相关的因子VIII可激活因子X。该反应的产物因子Xa随后可与毛细血管内皮细胞表达的类因子V分子相互作用，促进凝血酶形成。形成的凝血酶可裂解纤维蛋白原，导致纤维蛋白肽A释放和凝块形成。对内皮细胞进行内毒素处理会导致组织因子活性的诱导，在因子VIIa存在的情况下，该活性会促进因子IX和X的激活。与因子VIIa、IX、VIII和X一起孵育的经内毒素处理的内皮细胞形成的因子Xa量，比仅与因子VIIa和X孵育的细胞高8倍。这表明在受干扰的内皮细胞表面，因子VIII和IX在组织因子途径激活因子X的过程中确实发挥着重要作用。因此，受干扰的毛细血管内皮细胞可以提供一种血栓形成状态的模型，促进导致凝血酶形成的促凝途径的启动和传播。这种凝血途径依赖于内皮细胞，因为它需要毛细血管内皮细胞表达组织因子和因子V，以及凝血因子与毛细血管内皮细胞表面的相互作用。
