Suzuki Yoshimasa, Watanabe Satoshi, Odaira Mayumi, Okamura Toshiro, Kawata Yoshimasa
Appl Opt. 2019 Feb 1;58(4):954-962. doi: 10.1364/AO.58.000954.
Many studies have performed imaging under partially coherent illumination. However, to the best of our knowledge, there are no imaging methods for complicated objects such as cell colonies, which are large and diffract light multiple times. In this paper, we propose an image calculation method for the partially coherent illumination of a large-scale three-dimensional multi-diffractive object. The image is calculated via the summation of coherent images of each illumination angle using the beam propagation method. We apply this method to various microscopic observations, including phase contrast and differential interference contrast. Our method shows excellent agreement with experimental images of a bead and a photonic crystal fiber. As with a cell colony, our method reproduced the characteristics of the image through experimentation. Finally, we discuss the accuracy and the restriction conditions of our method.
许多研究在部分相干照明下进行成像。然而，据我们所知，对于诸如细胞集落等复杂物体，尚无成像方法，因为细胞集落体积大且会多次衍射光。在本文中，我们提出一种用于大规模三维多衍射物体部分相干照明的图像计算方法。该图像通过使用光束传播方法对每个照明角度的相干图像进行求和来计算。我们将此方法应用于各种微观观测，包括相衬和微分干涉衬度。我们的方法与珠子和光子晶体光纤的实验图像显示出极佳的一致性。对于细胞集落，我们的方法通过实验再现了图像的特征。最后，我们讨论了该方法的精度和限制条件。
