Wang P C, Chen S H
Biophys J. 1986 Jun;49(6):1205-14. doi: 10.1016/S0006-3495(86)83749-3.
A series of light scattering experiments have been performed to study both macroscopic aspects of band formation and propagation and microscopic motility parameters of Escherichia coli in the combined substrate gradients of oxygen and serine. From the band formation experiment the conclusion is drawn that a minimum threshold gradient of the substrate is required for bacteria to form a band. From the band propagation experiment in the serine substrate the motility coefficient mu and chemotactic coefficient delta are determined. A separate quasi-elastic scattering experiment has been made with a propagating band to obtain three microscopic motility parameters: mean twiddle time tau 1, mean run time tau 2, and mean run speed V2. Finally, a scaling argument is made to connect the macroscopic parameters mu and delta with the microscopic parameters tau 1, tau 2, and V2, thus achieving a unified understanding of macroscopic and microscopic aspect of chemotaxis.
已经进行了一系列光散射实验，以研究在氧气和丝氨酸的组合底物梯度中大肠杆菌条带形成和传播的宏观方面以及微观运动参数。从条带形成实验得出结论，细菌形成条带需要底物的最小阈值梯度。在丝氨酸底物中进行的条带传播实验确定了运动系数μ和趋化系数δ。对一个传播的条带进行了单独的准弹性散射实验，以获得三个微观运动参数：平均旋转时间τ1、平均游动时间τ2和平均游动速度V2。最后，进行了标度论证，将宏观参数μ和δ与微观参数τ1、τ2和V2联系起来，从而实现了对趋化性宏观和微观方面的统一理解。
