半乳糖基转移酶活性并非定位于人类小肠的刷状缘膜。
Galactosyltransferase activity is not localized to the brush border membrane of human small intestine.
作者信息
Boyle F, Snape S, Duane P, Cook N, Peters T
出版信息
Biosci Rep. 1986 Feb;6(2):171-5. doi: 10.1007/BF01115003.
A recent report [Roth et al. (1985) J. Cell Biol. 100: 118-125], using immunocytochemical techniques, claimed that human duodenal galactosyltransferase is located predominantly on the external aspect of enterocyte brush border membranes. Analytical subcellular fractionation by sucrose density gradient centrifugation of human jejunum biopsy homogenates demonstrated that galactosyltransferase activity is localized to the Golgi fraction (equilibrium density of 1.14 g cm-3) and is not found in significant amounts in the brush border membrane (equilibrium density of 1.22 g cm-3).
最近一份报告[罗斯等人（1985年），《细胞生物学杂志》100: 118 - 125]使用免疫细胞化学技术宣称，人十二指肠半乳糖基转移酶主要位于肠细胞刷状缘膜的外侧。通过对人空肠活检匀浆进行蔗糖密度梯度离心的亚细胞分级分离分析表明，半乳糖基转移酶活性定位于高尔基体部分（平衡密度为1.14 g/cm³），而在刷状缘膜（平衡密度为1.22 g/cm³）中未发现大量该酶。
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