Bourguet A, Desprez I, Volait L, Guyonnet A, Pignon C, Boulouis H J, Guillot J, Chahory S
Unité d'Ophtalmologie, École Nationale Vétérinaire d'Alfort, Université Paris - Est, Maisons-Alfort, F-94700, France.
Unité de Médecine des Animaux Exotiques, École Nationale Vétérinaire d'Alfort, Université Paris - Est, Maisons-Alfort, F-94700, France.
J Small Anim Pract. 2019 Jul;60(7):417-422. doi: 10.1111/jsap.12989. Epub 2019 Mar 15.
To determine the normal conjunctival bacterial and fungal flora in healthy rabbits, to compare the fungal flora from the conjunctival sac with periocular cutaneous fungal flora and to evaluate potential changes in type of micro-organisms over time.
Samples from 50 pet rabbits with a normal ophthalmic examination were obtained from conjunctival sacs using sterile cotton swabs and from the interpupillary skin space using a piece of sterile carpet. Samples were collected twice, 4 weeks apart, and cultured to evaluate bacterial and fungal populations.
Bacteria were recovered from 35.9% of conjunctival swabs. Corynebacterium species (32.4%), Staphylococcus species (18.3%), Pasteurella species (9.9%) and Moraxella species (8.5%) were the most frequently isolated organisms. Fungi were recovered from 14.6% of conjunctival swabs. The most common fungal genera were Aspergillus (37%), Scopulariopsis (14.8%) and Penicillium (11.1%). Fungal cultures from periocular integument yielded positive results in 96.3% of samples; the same fungal organism was isolated in both conjunctival and cutaneous culture in nine of 20 rabbits (45%). When comparing results 4 weeks apart in 32 of 50 animals, only three rabbits with a positive bacterial culture and one rabbit with a positive fungal culture had the same result at the subsequent test.
Fungi and bacteria isolated from the conjunctival sac of rabbits may represent transient seeding from the environment. To our knowledge, this is the first report of fungal composition of the conjunctival sac of rabbits and evaluation of the potential transient nature of both bacterial and fungal composition of this conjunctival flora.
确定健康兔正常结膜细菌和真菌菌群，比较结膜囊真菌菌群与眼周皮肤真菌菌群，并评估微生物类型随时间的潜在变化。
使用无菌棉拭子从50只眼科检查正常的宠物兔的结膜囊中获取样本，并使用一块无菌地毯从瞳孔间皮肤区域获取样本。样本分两次采集，间隔4周，进行培养以评估细菌和真菌数量。
从35.9%的结膜拭子中分离出细菌。棒状杆菌属（32.4%）、葡萄球菌属（18.3%）、巴斯德菌属（9.9%）和莫拉菌属（8.5%）是最常分离出的微生物。从14.6%的结膜拭子中分离出真菌。最常见的真菌属是曲霉属（37%）、帚霉属（14.8%）和青霉属（11.1%）。眼周皮肤的真菌培养在96.3%的样本中呈阳性结果；20只兔子中有9只（45%）在结膜和皮肤培养中分离出相同的真菌。在50只动物中的32只相隔4周比较结果时，只有3只细菌培养阳性的兔子和1只真菌培养阳性的兔子在后续测试中结果相同。
从兔结膜囊中分离出的真菌和细菌可能代表来自环境的短暂定植。据我们所知，这是关于兔结膜囊真菌组成以及对该结膜菌群细菌和真菌组成潜在短暂性质评估的首次报告。
