Lu Bolun, Wang Hao, Lu Qing, Tang Zhengya, Dou Hongjing, Dai Tingting, Li Shengli
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering
The State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University
J Comput Assist Tomogr. 2019 Mar/Apr;43(2):350-357. doi: 10.1097/RCT.0000000000000842.
To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).
Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions. After their characterization was validated, they were used to mapping SLNs by MRI in Wistar rats, and their biosafety was evaluated.
Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection). The in vivo T1-weighted MRI results revealed their effectiveness at mapping SLNs. And their biological safety was also verified.
Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.
评估一种新型的葡聚糖-钆纳米颗粒（NPs）作为高弛豫率T1磁共振成像（MRI）造影剂用于前哨淋巴结（SLN）定位的适用性。
通过自组装辅助方法合成葡聚糖-双丙烯酰胺-聚丙烯酸（Dex-MBA-PAA）纳米颗粒，并与多个螯合的钆（Gd）（III）离子络合。在对其特性进行验证后，将它们用于Wistar大鼠的MRI前哨淋巴结定位，并评估其生物安全性。
葡聚糖-MBA-聚丙烯酸-Gd纳米颗粒具有合适的粒径，并且纵向弛豫率（r1）比常用的临床MRI造影剂（如钆喷酸葡胺注射液）高得多。体内T1加权MRI结果显示了它们在前哨淋巴结定位中的有效性。并且它们的生物安全性也得到了验证。
通过体外和体内实验合成并验证了葡聚糖-MBA-聚丙烯酸-Gd纳米颗粒通过MRI可视化前哨淋巴结的能力，具有精确定位和出色的生物安全性，并且它们在临床前哨淋巴结定位方面具有巨大潜力。
