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利用超高效液相色谱-电喷雾电离四极杆飞行时间质谱联用仪(UPLC-ESI-Q-TOF-MS)研究油体和油橄榄果渣油中三酰基甘油的分布。

The Triacylglycerol Profile of Oil Bodies and Oil Extracted from Argania spinosa Using the UPLC Along with the Electrospray Ionization Quadrupole-Time-of-Flight Mass Spectrometry (LC-Q-TOF-MS).

机构信息

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan Univ., 1800 Lihu Ave., Wuxi, Jiangsu Province, 214122, PR China.

出版信息

J Food Sci. 2019 Apr;84(4):762-769. doi: 10.1111/1750-3841.14488. Epub 2019 Mar 15.

Abstract

The triacylglycerol (TAG) matrix of argan oil (AO) bodies (AOB) along with the TAGs of AO extracted from the same kernels using an organic solvent, were identified and quantified using the ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Generally, both samples showed a similar TAGs profile but AO found to have three extra TAGs in low amount. In total 23 and 26 different TAGs were identified in AOBs and AO, respectively. The most abundant TAGs were OOL, POO, OOO, and POL in both samples. Furthermore, oleic acid, linoleic acid, and palmitic acid were the major fatty acids in both AOBs and AO. To the best of our knowledge, this is the first research that studied the TAGs matrix of an oil body revealing no major difference between the TAGs profile protected by the AOBs membrane and the oil extracted from the whole seed. PRACTICAL APPLICATION: Seed and kernels oil bodies emulsion tend to be the new source of emulsified oil in food and cosmetic industries. However, before replacing a product with another, we have to make sure that the new alternative can offer better or at least similar benefits. Our results showed that the triacylglycerols (TAGs) matrix and the argan oil (AO) share the same TAGs profile with a relatively close percentage. Therefore, AO bodies can be the perfect pre-emulsified oil for some food products like sauces and creams.

摘要

采用超高效液相色谱-四级杆飞行时间质谱联用技术对阿甘油体(AOB)中的三酰基甘油(TAG)基质以及用有机溶剂从同一核仁中提取的阿甘油(TAG)进行了鉴定和定量分析。一般来说,这两种样品的TAGs 图谱相似,但阿甘油中发现有三种含量较低的额外 TAGs。在 AOBs 和 AO 中共鉴定出 23 种和 26 种不同的 TAGs。最丰富的 TAGs 是 OOL、POO、OOO 和 POL,这两种样品中均存在。此外,油酸、亚油酸和棕榈酸是 AOBs 和 AO 中主要的脂肪酸。据我们所知,这是首次研究油体的 TAGs 基质,揭示了油体膜保护的 TAGs 图谱与从整个种子中提取的油之间没有明显差异。实际应用:种子和核仁油体乳液可能成为食品和化妆品工业中乳化油的新来源。然而,在用一种产品替代另一种产品之前,我们必须确保新的替代品能提供更好的或至少相似的益处。我们的结果表明,三酰基甘油(TAGs)基质和阿甘油(AO)具有相同的 TAGs 图谱,且相对百分比相近。因此,AOB 可以成为某些食品(如酱汁和面霜)的理想预乳化油。

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