The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemical and Materials Science, Shanghai Normal University, Shanghai 200234, PR China.
College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
Biosens Bioelectron. 2019 May 1;132:203-209. doi: 10.1016/j.bios.2019.03.004. Epub 2019 Mar 6.
A stable sandwiched electrochemiluminescence (ECL) aptasensor was originally constructed established upon Ru(bpy)-doped silica nanoparticles (RuSiO NPs) with ferrocene carboxylic acid-aptamer (Fc-aptamer) to quantitatively detect cytochrome c (Cyt C). Herein, RuSiO NPs and Fc-aptamer were respectively prepared through the microemulsion method and amide reaction to fabricate the ECL aptasensor. Furthermore, Fc-aptamer was used as quenching probe for quenching the ECL emission of RuSiO NPs. In detail, RuSiO NPs were primarily immobilized onto the electrodes by the film-forming function of chitosan. Subsequently, the aptamer was incubated onto the decorated GCE via crosslinking with glutaraldehyde (GA). After Cyt C was connected to the GCE via immunoreaction, Fc-aptamer was immobilized onto the modified electrodes owing to the specific recognition between antigens and aptamer. Ultimately, ECL signals markedly descended owing to the poor electricity conductivity of proteins and superior quenching effect of Fc-aptamer. Under optimum conditions, the designed ECL aptasensor indicated an accurate analysis for Cyt C in a rang of 0.001-100 nM with a detection limit of 0.48 pM (S/N = 3).
建立了一种稳定的夹心电化学发光（ECL）适体传感器，该传感器基于钌（bpy）掺杂的硅纳米粒子（RuSiO NPs）和二茂铁羧酸适体（Fc-aptamer），用于定量检测细胞色素 c（Cyt C）。在此，通过微乳液法和酰胺反应分别制备了 RuSiO NPs 和 Fc-aptamer，以构建 ECL 适体传感器。此外，Fc-aptamer 被用作猝灭探针来猝灭 RuSiO NPs 的 ECL 发射。具体而言，通过壳聚糖的成膜作用将 RuSiO NPs 最初固定在电极上。随后，通过与戊二醛（GA）交联将适体孵育到修饰的 GCE 上。在 Cyt C 通过免疫反应连接到 GCE 后，由于抗原和适体之间的特异性识别，Fc-aptamer 被固定在修饰的电极上。最终，由于蛋白质的导电性差和 Fc-aptamer 的优异猝灭效应，ECL 信号明显下降。在最佳条件下，所设计的 ECL 适体传感器在 0.001-100 nM 的范围内对 Cyt C 进行了准确分析，检测限为 0.48 pM（S/N=3）。
