一种新型的通过整合 HCR 信号放大和 ECL 银簇来分析组蛋白乙酰转移酶 (HATs) 活性的 ECL 方法。
A novel ECL method for histone acetyltransferases (HATs) activity analysis by integrating HCR signal amplification and ECL silver clusters.
机构信息
Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Kay Lab of Bioorganic Phosphorus Chemistry and Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China; Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong 266071, China.
Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong 266071, China.
出版信息
Talanta. 2019 Jun 1;198:39-44. doi: 10.1016/j.talanta.2019.01.083. Epub 2019 Jan 25.
A novel electrogenerated chemiluminescence (ECL) method combining ECL property of silver clusters and hybridization chain reaction (HCR) signal amplification strategy has been designed for the analysis of histone acetyltransferases (HATs) activity and inhibitor evaluation. In this strategy, the substrate peptide of HAT released from the electrode surface due to the charge change based on the acetylated reaction in the presence of HATs, and then the exposed DNA on the electrode initiated the HCR to form the supersandwich DNA sequence, which can adsorb Ag, and the silver clusters (AgNCs) generated by the electrochemical reduction. The ECL signal generated by AgNCs can be utilized for HATs activities detection. The detection limit of the as-prepared ECL method was 0.49 nM (S/N = 3). The novel ECL method can be used for HATs activity analysis and inhibition in MCF-7 cell lysates which shows high promise in HATs-related clinical diagnostics.
一种新型的电致化学发光（ECL）方法，结合了银团的 ECL 性质和杂交链式反应（HCR）信号放大策略，被设计用于分析组蛋白乙酰转移酶（HATs）的活性和抑制剂评估。在该策略中，由于 HATs 存在下的乙酰化反应，底物肽从电极表面释放出来，然后暴露在电极上的 DNA 引发 HCR 形成超三明治 DNA 序列，该序列可以吸附 Ag，以及通过电化学还原生成的银团簇（AgNCs）。AgNCs 产生的 ECL 信号可用于检测 HATs 的活性。所制备的 ECL 方法的检测限为 0.49 nM（S/N=3）。新型 ECL 方法可用于 MCF-7 细胞裂解物中的 HATs 活性分析和抑制，这在 HATs 相关的临床诊断中具有很大的应用前景。
Zotero 插件