Kapoor A, Batra J K, Luthra U K, Jain R C, Venkitasubramanian T A, Raj H G
Eur J Drug Metab Pharmacokinet. 1986 Jan-Mar;11(1):33-7. doi: 10.1007/BF03189773.
Sputum collected from patients with respiratory diseases were examined for presence of benzo(a)pyrene hydroxylase (BPH) activity. The human bronchial mucus used in these studies had significant capability to metabolize benzo(a)pyrene. Clarification of the sputum by agents such as N-acetylcysteine or pancreatin in presence of antibiotics was found to be essential for the detection of BPH activity. In vitro incubation of the clarified human bronchial mucus with benzoflavone caused inhibition, while 7,8-dimethyl-benzanthracene induced BPH enzyme activity.
对患有呼吸系统疾病患者咳出的痰液进行检测，以确定其中是否存在苯并(a)芘羟化酶（BPH）活性。这些研究中使用的人支气管黏液具有显著的代谢苯并(a)芘的能力。发现在抗生素存在的情况下，使用N-乙酰半胱氨酸或胰酶等试剂对痰液进行澄清，对于检测BPH活性至关重要。将澄清的人支气管黏液与苯并黄酮进行体外孵育会导致抑制作用，而7,8-二甲基苯并蒽则会诱导BPH酶活性。
