Schneider K, Beck C F
Gene. 1986;42(1):37-48. doi: 10.1016/0378-1119(86)90148-4.
A series of plasmid-based promoter-probe vectors has been constructed which are particularly useful for the analysis of divergent control regions. Each vector contains a pair of divergently oriented indicator genes whose expression can be monitored over a wide range by simple assay methods. These genes are separated by different polylinkers. Specifically, the beta-galactosidase gene (lacZ) was employed in combination with either the galactokinase gene (galK) or the alkaline phosphatase gene (phoA). In all cases translational stop codons are present in all three reading frames upstream from the initiation codon. The vectors permit direct detection of promoters--independent of insert orientation--on indicator plates after transformation. Using this vector system, we further characterized the divergent tet control regions of transposon Tn10 and plasmid pBR322.
已经构建了一系列基于质粒的启动子探针载体，这些载体对于分析双向控制区特别有用。每个载体都包含一对反向排列的指示基因，其表达可以通过简单的检测方法在很宽的范围内进行监测。这些基因由不同的多克隆位点隔开。具体而言，β-半乳糖苷酶基因（lacZ）与半乳糖激酶基因（galK）或碱性磷酸酶基因（phoA）结合使用。在所有情况下，起始密码子上游的所有三个阅读框中都存在翻译终止密码子。这些载体允许在转化后在指示平板上直接检测启动子，而与插入方向无关。使用这个载体系统，我们进一步表征了转座子Tn10和质粒pBR322的双向四环素控制区。
