Burnett A M, Ezzell J W, Singh J, Zipperle G F, Doyle R J
J Appl Bacteriol. 1986 Apr;60(4):337-9. doi: 10.1111/j.1365-2672.1986.tb01741.x.
Incubation of sporulating cultures of Bacillus anthracis, B. cereus, B. subtilis and B. thuringiensis in 1.0 mol/l sodium sulphate markedly increased the release of free spores from sporangia. It is postulated that the release of spores is due to activation of latent autolysins which hydrolyse sporangial cell walls. Sodium sulphate-induced lysis of sporangia represents a novel and highly effective method for the recovery of spores from cultures of Bacillus species.
将炭疽芽孢杆菌、蜡样芽孢杆菌、枯草芽孢杆菌和苏云金芽孢杆菌的产孢培养物在1.0摩尔/升硫酸钠中培养，可显著增加游离芽孢从孢子囊中的释放。据推测，芽孢的释放是由于潜在自溶素的激活，自溶素可水解孢子囊细胞壁。硫酸钠诱导的孢子囊裂解是一种从芽孢杆菌属培养物中回收芽孢的新颖且高效的方法。
