Kyriakidis Theofylaktos, Iosifidis Michael, Michalopoulos Efstathios, Melas Ioannis, Papadopoulos Pericles, Stavropoulos-Giokas Catherine
Acta Orthop Belg. 2018 Dec;84(4):443-451.
The present study aims to evaluate the efficacy of matrix-induced adipose-derived mesenchymal stem cells (Ad-MSCs) for cartilage repair of focal chondral knee lesions. Twenty patients were initially treated for symptomatic full-thickness chondral defects and then prospectively followed for two years. All patients underwent a single- stage procedure consisting in filling each defect with autologous culture-expanded mesenchymal stem cells embedded in a trimmed-to-fit commercially available biodegradable matrix. Knee-related function was evaluated based on subjective scores given by two self-reported questionnaires (KOOS and IKDC). Data analysis shows significant improvements (p<0.001) in all values. The mean preoperative scores in the subscales of KOOS, as well as in the IKDC subjective score were constantly increased during the follow-up period with statistically significant differences at 6, 12 and 24 months follow-up. The findings of this study indicate that matrix- induced adipose-derived mesenchymal stem cells implantation is an effective and safe single-staged cell-based procedure to manage full-thickness focal chondral lesions of the knee.
本研究旨在评估基质诱导的脂肪来源间充质干细胞（Ad-MSCs）对膝关节局灶性软骨损伤修复的疗效。20例患者最初接受有症状的全层软骨缺损治疗，然后进行为期两年的前瞻性随访。所有患者均接受单阶段手术，即使用嵌入经修剪以适合的市售可生物降解基质中的自体培养扩增间充质干细胞填充每个缺损。基于两份自我报告问卷（KOOS和IKDC）给出的主观评分评估膝关节相关功能。数据分析显示所有数值均有显著改善（p<0.001）。在随访期间，KOOS各子量表的术前平均评分以及IKDC主观评分持续增加，在6个月、12个月和24个月随访时有统计学显著差异。本研究结果表明，基质诱导的脂肪来源间充质干细胞植入是一种有效且安全的单阶段基于细胞的治疗膝关节全层局灶性软骨损伤的方法。
