Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, 100048, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
Carbohydr Polym. 2019 Jun 1;213:59-69. doi: 10.1016/j.carbpol.2019.02.080. Epub 2019 Feb 23.
Immunostimulatory activity of the flaxseed gum neutral fraction (NFG) was investigated. NFG was characterized as a xylose rich heteroglycan through monosaccharide composition analysis, FT-IR, methylation/GC-MS, and 1D/2D-NMR. NFG stimulated NO production and phagocytic activity of macrophages. Secretion of interleukin-6, interleukin-1β, and tumor necrosis factor-α (254.7 pg/mL, 2.5 ng/mL, and 42.9 pg/mL, respectively) was significantly induced by NFG. Mitogen-activated protein kinases of JNK and P38 were activated by NFG with increased phosphorylation of JNK and P38, while NO production was reduced to 6.05 and 4.42 μM by JNK and P38 inhibitor, respectively. Nuclear factor-κB signaling pathway was also activated by NFG with the suppression of IκBα and up-regulation of phosphorylation of IκBα and nuclear factor-κB P65. Toll like receptor-2 was the molecular target of NFG and responsible for the activation of down-stream signaling pathways. Thus, NFG from flaxseed gum may potentially be used as a natural immunomodulator in functional foods.
研究了亚麻籽胶中性部分（NFG）的免疫刺激活性。通过单糖组成分析、FT-IR、甲基化/GC-MS 和 1D/2D-NMR，将 NFG 表征为富含木糖的杂多糖。NFG 可刺激巨噬细胞产生 NO 和吞噬活性。NFG 可显著诱导白细胞介素-6、白细胞介素-1β 和肿瘤坏死因子-α 的分泌（分别为 254.7 pg/mL、2.5 ng/mL 和 42.9 pg/mL）。NFG 激活了丝裂原活化蛋白激酶 JNK 和 P38，导致 JNK 和 P38 的磷酸化增加，而 JNK 和 P38 抑制剂可将 NO 产生分别降低至 6.05 和 4.42 μM。NFG 还激活了核因子-κB 信号通路，抑制了 IκBα 的表达，并上调了 IκBα 和核因子-κB P65 的磷酸化。Toll 样受体-2 是 NFG 的分子靶标，负责激活下游信号通路。因此，亚麻籽胶 NFG 可能有望作为功能性食品中的天然免疫调节剂。
