Busso N, Belin D, Failly-Crépin C, Vassalli J D
J Biol Chem. 1986 Jul 15;261(20):9309-15.
Culture of human mammary HBL-100 cells in the presence of dexamethasone, a synthetic glucocorticoid, resulted in opposite effects on the production of the two plasminogen activators (PAs): a decrease in urokinase-type PA (u-PA) and a concomitant increase in tissue-type PA (t-PA). Two PA-specific inhibitors, one related to that produced by bovine aortic endothelial cells, and the other related to that isolated from human placenta, were also produced by these cells; dexamethasone did not affect the production of either of these inhibitors. The glucocorticoid effects observed on PA enzymatic activities were associated with changes in PA mRNA levels. Experiments using inhibitors of RNA and protein synthesis suggested that the glucocorticoid-induced decrease in u-PA mRNA was a secondary event, requiring synthesis of new regulatory proteins; in contrast, the increase in t-PA mRNA appeared to be a direct effect on t-PA gene expression.
在合成糖皮质激素地塞米松存在的情况下培养人乳腺HBL - 100细胞，对两种纤溶酶原激活剂（PA）的产生产生了相反的影响：尿激酶型PA（u - PA）减少，同时组织型PA（t - PA）增加。这些细胞还产生了两种PA特异性抑制剂，一种与牛主动脉内皮细胞产生的抑制剂相关，另一种与从人胎盘中分离出的抑制剂相关；地塞米松不影响这两种抑制剂中任何一种的产生。观察到的糖皮质激素对PA酶活性的影响与PA mRNA水平的变化有关。使用RNA和蛋白质合成抑制剂的实验表明，糖皮质激素诱导的u - PA mRNA减少是一个次级事件，需要合成新的调节蛋白；相反，t - PA mRNA的增加似乎是对t - PA基因表达的直接影响。
