State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, 999078, China.
Zhuhai Livzon Cynvenio Diagnostics Ltd., Zhuhai, Guangdong, 519060, China.
J Ethnopharmacol. 2019 Jun 12;237:20-27. doi: 10.1016/j.jep.2019.03.035. Epub 2019 Mar 14.
ETHNOPHARMACOLOGICAL RELEVANCE: The whole plant of Glycine tabacina (Labill.) Benth has been used as a traditional herbal medicine to treat rheumatism, ostealgia and nephritis in China. It is also one of the sources of the renowned native herbal medicine 'I-Tiao-Gung' in Taiwan. AIM OF THE STUDY: This study aimed to investigate the anti-arthritic effect of ethanol extract of G. tabacina (GTE) in a collagen-induced arthritis (CIA) rat model. MATERIALS AND METHODS: The chemical profile of GTE was analyzed by HPLC-UV. The CIA was induced in male Wistar rats by intradermal injection of bovine type II collagen at tail root, back and ankle joints. The rats were orally administrated daily with GTE (1.11, 2.22 and 4.44 g dry weight of herb powder per kg body weight) from day 0 and continued for 30 days. Swelling volume and thickness of paw, arthritis index, X-radiographs and histopathological changes were examined to assess the severity of arthritis. Furthermore, the levels of pro-inflammatory cytokines, such as interleukin1β (IL-1β), IL-6 and tumor necrosis factor α (TNF-α), total superoxide dismutase (T-SOD) activity and malonaldehyde (MDA) level were measured to preliminarily explore the possible mechanisms. RESULTS: Oral administration of GTE significantly ameliorated the arthritic symptoms in CIA rat model, as indicated by the effects on paws swelling and arthritis index. X-radiographic analysis and histopathological examinations demonstrated that GTE effectively protected the bone and cartilage of joints from erosion, lesion and deformation. The efficacy of GTE treatment on CIA was comparable to that of indomethacin (positive drug). Besides, the overproduction of IL-1β, IL-6 and TNF-α was remarkably inhibited in the serum of all GTE treatment groups. The restoration of serum T-SOD activity and MDA level proved that GTE administration alleviated the oxidative stress in CIA rats. CONCLUSIONS: GTE exhibited strong anti-CIA activity through inhibiting pro-inflammatory cytokines and oxidation in rats, suggesting its potential preventive and therapeutic effects on rheumatoid arthritis (RA).
民族药理学相关性：菝葜（Labill.）Benth 的全植物被用作传统草药，在中国用于治疗风湿、骨痛和肾炎。它也是台湾著名土生草药“一條根”的来源之一。
研究目的：本研究旨在探讨菝葜乙醇提取物（GTE）在胶原诱导性关节炎（CIA）大鼠模型中的抗关节炎作用。
材料和方法：采用 HPLC-UV 分析 GTE 的化学成分。通过在尾根、背部和踝关节皮内注射牛 II 型胶原诱导雄性 Wistar 大鼠 CIA。从第 0 天开始，大鼠每天口服 GTE（1.11、2.22 和 4.44 g 干重草药粉/公斤体重），连续 30 天。检查爪肿胀体积和厚度、关节炎指数、X 射线照片和组织病理学变化，以评估关节炎的严重程度。此外，还测量了促炎细胞因子（如白细胞介素 1β（IL-1β）、IL-6 和肿瘤坏死因子 α（TNF-α））、总超氧化物歧化酶（T-SOD）活性和丙二醛（MDA）水平的水平，初步探讨可能的机制。
结果：口服 GTE 显著改善 CIA 大鼠模型的关节炎症状，表现在爪肿胀和关节炎指数方面。X 射线分析和组织病理学检查表明，GTE 有效地保护关节的骨和软骨免受侵蚀、损伤和变形。GTE 治疗 CIA 的疗效与消炎痛（阳性药物）相当。此外，所有 GTE 治疗组的血清中 IL-1β、IL-6 和 TNF-α 的过度产生均显著受到抑制。血清 T-SOD 活性和 MDA 水平的恢复证明 GTE 给药减轻了 CIA 大鼠的氧化应激。
结论：GTE 通过抑制大鼠促炎细胞因子和氧化作用表现出强大的抗 CIA 活性，表明其对类风湿关节炎（RA）具有潜在的预防和治疗作用。
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