College of Pharmacy, Wannan Medical College, Wuhu, Anhui, China.
College of Life Science, Anhui Normal University, Wuhu, Anhui, China.
Pharm Biol. 2020 Dec;58(1):528-537. doi: 10.1080/13880209.2020.1767159.
[(Thunb.) Roem. et Schult, (Chloranthaceae)] is a folk medicine used for the treatment of rheumatoid arthritis. The aim of this study was to investigate anti-arthritic effects of the ethanol extracts of the roots (ER), stems (ES) and leaves (EL) of on adjuvant arthritis rats and related mechanisms. The rats were immunized by intradermal injection of complete Freund's adjuvant (CFA, 0.18 mL) into the right hind feet, and received intragastric administrations of the ER, ES and EL (2.07, 1.61 and 0.58 g/kg/d, respectively) for 14 days. The anti-arthritic activity was assessed by swelling rates, serum indicators, antioxidant capacity, histopathological and immunohistochemical analyses. The LD of the ER, ES and EL was higher than 10.35, 8.05 and 2.90 g/kg/p.o., respectively. Extract treatments decreased swelling rates, tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), interleukin 1 beta (IL-1β), migration inhibitory factor 1 (MIF-1), immunoglobulin G (IgG) and immunoglobulin M (IgM) levels and positive expression of VEGF in the arthritic rats ( < 0.01 or < 0.05). The ER significantly decreased NO (3.91 ± 0.61 µmol/L), IL-6 (75.67 ± 16.83 pg/mL) and malondialdehyde (MDA) (2.28 ± 0.32 nmol/mL) contents and clearly increased IFN-γ (2082 ± 220.93 pg/mL) and superoxide dismutase (SOD) (601.98 ± 38.40 U/mL) levels. The ES and EL did not reverse the changes in some indicators. All the extracts alleviated inflammatory cell infiltration and synovial cell proliferation. Among them, the ER was the most pronounced. ER exerts the most promising effects, as shown by inhibiting the releases of inflammatory cytokines and enhancing antioxidant capacity, which provides a scientific basis for further research on and its clinical applications.
[醉鱼草（Thunb.）Roem. et Schult.，（醉鱼草科）]是一种民间药物，用于治疗类风湿关节炎。本研究旨在探讨根（ER）、茎（ES）和叶（EL）的乙醇提取物对佐剂关节炎大鼠的抗关节炎作用及相关机制。大鼠通过皮内注射完全弗氏佐剂（CFA，0.18mL）于右后足进行免疫，连续灌胃给药 14 天，剂量分别为 ER（2.07g/kg/d）、ES（1.61g/kg/d）和 EL（0.58g/kg/d）。通过肿胀率、血清指标、抗氧化能力、组织病理学和免疫组织化学分析来评估抗关节炎活性。ER、ES 和 EL 的 LD 均高于 10.35、8.05 和 2.90g/kg/po。提取物治疗降低了肿胀率、肿瘤坏死因子-α（TNF-α）、血管内皮生长因子（VEGF）、白细胞介素 1β（IL-1β）、迁移抑制因子 1（MIF-1）、免疫球蛋白 G（IgG）和免疫球蛋白 M（IgM）水平以及 VEGF 的阳性表达（ < 0.01 或 < 0.05）。ER 显著降低了 NO（3.91±0.61μmol/L）、IL-6（75.67±16.83pg/mL）和丙二醛（MDA）（2.28±0.32nmol/mL）的含量，并明显增加了 IFN-γ（2082±220.93pg/mL）和超氧化物歧化酶（SOD）（601.98±38.40U/mL）的水平。ES 和 EL 并未逆转部分指标的变化。所有提取物均减轻了炎症细胞浸润和滑膜细胞增殖。其中，ER 的作用最为明显。ER 表现出最有希望的效果，表现为抑制炎症细胞因子的释放和增强抗氧化能力，为进一步研究 及其临床应用提供了科学依据。
