Butler B D
J Clin Ultrasound. 1986 Jun;14(5):408-12. doi: 10.1002/jcu.1870140517.
A variety of agents have been proposed for use as contrast for ultrasound studies. Several reports suggest the use of microbubbles because of their superior sonographic resolution. We describe a method of producing calibrated microbubbles utilizing differential absorption and gas injection techniques. Gas injection methods are described using a Plexiglas microbubbler apparatus with a fine needle orifice. The bubbles originally produced by this device are in a size range of 40-100 microns and consist of nitrogen (10%) and carbon dioxide (90%). The differential absorption technique uses THAM [tris(hydroxymethyl)aminomethane hydrochloride] to absorb the carbon dioxide (diluent gas) from the bubbles selectively, leaving smaller microbubbles. The microbubbles can be calibrated using the electric gating principle of the Coulter Counter.
已有多种试剂被提议用作超声研究的造影剂。一些报告建议使用微泡，因为它们具有卓越的超声分辨率。我们描述了一种利用差分吸收和气体注入技术生产校准微泡的方法。气体注入方法是使用带有细针孔的有机玻璃微泡发生器装置来描述的。该装置最初产生的气泡大小在40 - 100微米范围内，由氮气（10%）和二氧化碳（90%）组成。差分吸收技术使用三羟甲基氨基甲烷盐酸盐（THAM）选择性地从气泡中吸收二氧化碳（稀释气体），从而留下较小的微泡。微泡可以使用库尔特计数器的电门控原理进行校准。
