通过联合碱性磷酸酶抗碱性磷酸酶（APAAP）染色和放射自显影术鉴定增殖淋巴细胞亚群。

Identification of proliferating lymphocyte subpopulations by combined alkaline phosphatase anti-alkaline phosphatase (APAAP) staining and autoradiography.

作者信息

Schneider H, Wachner R

出版信息

J Immunol Methods. 1986 Jun 24;90(2):283-5. doi: 10.1016/0022-1759(86)90086-4.

Abstract

An improvement in the classification of proliferating ([3H]thymidine incorporating) lymphocyte subpopulations in mitogen- or antigen-stimulated microcultures is described. The binding of subset-specific monoclonal antibodies is detected by the alkaline phosphatase anti-alkaline phosphatase method (APAAP). There are two advantages compared to the peroxidase anti-peroxidase (PAP) method; (1) endogenous enzyme (peroxidase) activity exhibited by some cells causes no interference, and (2) the red alkaline phosphatase staining obtained with new fuchsin provides a far superior contrast to silver grains than conventional peroxidase staining.

摘要

本文描述了在丝裂原或抗原刺激的微量培养物中增殖性（掺入[3H]胸腺嘧啶核苷）淋巴细胞亚群分类的改进方法。通过碱性磷酸酶抗碱性磷酸酶法（APAAP）检测亚群特异性单克隆抗体的结合。与过氧化物酶抗过氧化物酶（PAP）法相比，有两个优点：（1）某些细胞表现出的内源性酶（过氧化物酶）活性不会产生干扰；（2）用新番红获得的红色碱性磷酸酶染色与银颗粒相比，比传统的过氧化物酶染色提供了更优越的对比度。