通过表面标志物和放射自显影术鉴定微量培养物中增殖的淋巴细胞亚群。
Identification of proliferating lymphocyte subpopulations in microcultures by surface marker and autoradiography.
作者信息
Schneider H, Vogt A, Bross K
出版信息
Immunol Commun. 1984;13(6):553-61. doi: 10.3109/08820138409061306.
PMID:6241922
Abstract
A micromethod is described which allows subpopulation classification of proliferating (3H thymidine incorporating) cells, previously stimulated in microcultures. The technique is based on transferring 1,000 to 5,000 cells from microcultures to poly-L-lysine coated multispot slides. The cells are then stained for surface markers using the immuno-peroxidase method, combined with subsequent autoradiography.
摘要
本文描述了一种微量方法，该方法可对先前在微量培养中受到刺激的增殖（掺入3H胸腺嘧啶核苷）细胞进行亚群分类。该技术基于将1000至5000个细胞从微量培养物转移到聚-L-赖氨酸包被的多斑点载玻片上。然后使用免疫过氧化物酶方法对细胞进行表面标志物染色，并结合随后的放射自显影。
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