Wang Huan, Wei Hongling, Tang Lei, Lu Junkai, Mu Changkao, Wang Chunlin
School of Marine Science, Ningbo University, Ningbo, 315211 Zhejiang, China.
Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, 315211 Zhejiang, China.
Int J Genomics. 2019 Feb 10;2019:7940405. doi: 10.1155/2019/7940405. eCollection 2019.
is a commercially important species distributed along the coast of southern China and other Indo-Pacific countries. Sudden salinity drop exceeding the adjustment capability of can result in damage or even mortality. In our previous study, we had analyzed the mechanism of adapting sudden drop in salinity from the level of transcriptomics and proteomics, respectively. This study performed a correlation analysis of RNA sequencing transcriptomics and iTRAQ proteomics in order to investigate the adaptation mechanisms to sudden salinity drop from 23‰ to 3‰. There were 3954 correlations and a total of 15 correlations for differentially expressed proteins (DEPs) and differentially expressed genes (DEGs) from proteomics and transcriptomics. The correlation between DEPs and DEGs was 0, and the Spearman correlation coefficient of the same trend correlation for DEPs and DEGs was the highest, reaching 0.9080. KEGG pathway enrichment correlation revealed that protein digestion and absorption (Ko04974), proximal tubule bicarbonate (Ko04964), and bile secretion (Ko04976) played important roles in Na/H and Na/K exchange. In addition, important genes related to osmoregulation, such as ion transport and carbonic anhydrase, were also detected in the correlation analysis for same trend DEPs_DEGs. In conclusion, the proteome and transcriptome correlation results from this study indicate that ion transport plays a critical role in the adaptation of to sudden reduction in salinity.
是一种具有重要商业价值的物种，分布于中国南部沿海及其他印度-太平洋国家。盐度突然下降超过其调节能力会导致损害甚至死亡。在我们之前的研究中，我们分别从转录组学和蛋白质组学水平分析了适应盐度突然下降的机制。本研究对RNA测序转录组学和iTRAQ蛋白质组学进行了相关性分析，以探究对盐度从23‰突然降至3‰的适应机制。蛋白质组学和转录组学的差异表达蛋白（DEPs）和差异表达基因（DEGs）之间存在3954个相关性，共15个相关性。DEPs和DEGs之间的相关性为0，DEPs和DEGs相同趋势相关性的Spearman相关系数最高，达到0.9080。KEGG通路富集相关性显示，蛋白质消化与吸收（Ko04974）、近端小管碳酸氢盐（Ko04964）和胆汁分泌（Ko04976）在Na/H和Na/K交换中起重要作用。此外，在相同趋势DEPs_DEGs的相关性分析中还检测到与渗透调节相关的重要基因，如离子转运和碳酸酐酶。总之，本研究的蛋白质组和转录组相关性结果表明，离子转运在适应盐度突然降低中起关键作用。
