小鼠IgG抗体亚类对日本血吸虫童虫的杀伤作用
The killing effect of subclasses of mouse IgG antibodies on schistosomula of Schistosoma japonicum.
作者信息
Maeda S, Irie Y, Ichii S, Yasuraoka K
出版信息
Jpn J Exp Med. 1986 Feb;56(1):27-33.
The killing effect of IgG antibodies in sera from S. japonicum infected mice on schistosomula of S. japonicum was studied in vitro in the presence of complement. Immune mouse sera were fractionated over Protein A-Sepharose and IgG1, IgG2a, IgG2b and IgG3 were purified by affinity chromatography. The killing activity of each subclass of IgG antibodies against schistosomula was observed only in the presence of complement. The activity of IgG1 was higher than that of the other subclasses of IgG. In combination of the two or three subclasses of IgG, the additive mortality rate was given in all combinations of each subclass of IgG. These results support the view that the combination of various subclasses of IgG antibodies potentiates the resultant lethal effect on schistosomula.
在补体存在的情况下，体外研究了日本血吸虫感染小鼠血清中IgG抗体对日本血吸虫童虫的杀伤作用。将免疫小鼠血清通过蛋白A-琼脂糖凝胶进行分离，并用亲和层析法纯化IgG1、IgG2a、IgG2b和IgG3。仅在补体存在时观察到各IgG抗体亚类对童虫的杀伤活性。IgG1的活性高于其他IgG亚类。在IgG的两个或三个亚类组合中，各IgG亚类的所有组合均呈现相加死亡率。这些结果支持以下观点，即IgG抗体的各种亚类组合可增强对童虫的最终致死作用。
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