靶向触发的动态发夹组装用于 miRNA 和癌基因的信号放大及其在活细胞成像中的应用。
Target-triggered dynamic hairpin assembly for signal amplification of microRNA and oncogenes and its application in live-cell imaging.
机构信息
University-Industry Joint Center for Ocean Observation and Broadband Communication, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, P. R. China.
出版信息
Chem Commun (Camb). 2019 Apr 2;55(28):4103-4106. doi: 10.1039/c9cc00747d.
PMID:30887983
Abstract
Based on target-triggered dynamic hairpin assembly (DHA) in both unidirectional and bilateral growth manners DNA nanobrushes are constructed, which realize sensitive and selective detection of short miRNA (miR-21) and long DNA (BRCA1), respectively. Moreover, the unidirectional DHA strategy is readily applied to in situ imaging of miR-21 in different live cells.
摘要
基于在单向和双向生长方式下的靶触发动态发夹组装（DHA），构建了 DNA 纳米刷，分别实现了对短 miRNA（miR-21）和长 DNA（BRCA1）的灵敏和选择性检测。此外，还将单向 DHA 策略应用于不同活细胞中 miR-21 的原位成像。
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