Kühn F, Wolff H, Hahn H J, Lorenz D, Lippert H, Schulz H J, Gütte N
Zentralbl Chir. 1986;111(9):556-64.
Preservation of B-cells, following organ collection and cold ischaemia, was defined on 19 donor organs by means of histological tests and testing for insulin content in pancreatic tissue. The degree of preservation declined by 50 per cent after two hours of cold ischaemia. The Velcro technique and uncrushed organ digestion were used for isolation. Islet yield was calculated by determination of insulin levels in isolated tissue (nmol/ml) and in the islet (pmol/islet). The average islet yield was 2 076 islets to one gram pancreas, that is ten per cent of the original amount. Studies conducted into in-vitro insulin secretion from isolated islets have revealed damage to organs due to handling, especially to organs which had undergone extended cold ischaemia.
在器官采集和冷缺血后，通过组织学检测和胰腺组织胰岛素含量检测，对19个供体器官的B细胞保存情况进行了界定。冷缺血两小时后，保存程度下降了50%。采用尼龙搭扣技术和未挤压器官消化法进行分离。通过测定分离组织（nmol/ml）和胰岛（pmol/胰岛）中的胰岛素水平来计算胰岛产量。平均每克胰腺的胰岛产量为2076个胰岛，即原始数量的10%。对分离胰岛的体外胰岛素分泌进行的研究表明，操作会对器官造成损伤，尤其是经历了长时间冷缺血的器官。
