Takai Y, Noda Y, Sumitomo S, Sagara S, Mori M
Acta Histochem. 1986;78(2):111-21. doi: 10.1016/s0065-1281(86)80041-1.
Lectin binding affinities were described in human submandibular gland (SMG) in the paraffin sections following alpha-amylase, sialidase, and trypsin digestions. Lectins in the present study were used Con A (Glc, Man binding lectins), PNA, and SBA(Gal, GalNAc), RCA-1(Gal), DBA(GalNAc), WGA(GlcNAc), and UEA-1(Fuc). Lectin stainings in serous and mucous acinar cells and ductal epithelia were reported to compare enzyme treated and nontreated sections. Amylase treatment showed increasing Con A staining in connective tissue fibers and no marked changes in SMG to lectin bindings. Sialidase digestion was characteristically intense in PNA and SBA bindings in SMG cells, and also enhanced staining to UEA-1 in serous and duct cells and to WGA in mucous and duct cells were noted. Trypsin digestion indicated a slight increase to Con A binding, and was relatively strong to UEA-1 in serous and duct cells and a little strong to WGA. The results suggested that SMG serous cells contain higher amounts of Gal, GalNAc, and Fuc residues; and mucous cells were also abundant in Gal, GalNAc, and GlcNAc residues.
在α-淀粉酶、唾液酸酶和胰蛋白酶消化后的石蜡切片中，描述了人下颌下腺（SMG）中的凝集素结合亲和力。本研究中使用的凝集素为刀豆球蛋白A（Con A，结合葡萄糖、甘露糖的凝集素）、花生凝集素（PNA）、大豆凝集素（SBA，结合半乳糖、N-乙酰半乳糖胺）、蓖麻凝集素-1（RCA-1，结合半乳糖）、荆豆凝集素（DBA，结合N-乙酰半乳糖胺）、小麦胚凝集素（WGA，结合N-乙酰葡糖胺）和荆豆凝集素-1（UEA-1，结合岩藻糖）。报告了浆液性腺泡细胞、黏液性腺泡细胞和导管上皮细胞中的凝集素染色情况，以比较酶处理组和未处理组切片。淀粉酶处理显示结缔组织纤维中刀豆球蛋白A染色增加，而下颌下腺对凝集素结合无明显变化。唾液酸酶消化在下颌下腺细胞的花生凝集素和大豆凝集素结合中特征性地强烈，并且还注意到浆液性腺泡细胞和导管细胞中对荆豆凝集素-1的染色增强，黏液性腺泡细胞和导管细胞中对小麦胚凝集素的染色增强。胰蛋白酶消化表明刀豆球蛋白A结合略有增加，浆液性腺泡细胞和导管细胞中对荆豆凝集素-1的结合相对较强，对小麦胚凝集素的结合略有增强。结果表明，下颌下腺浆液性腺泡细胞含有较高量的半乳糖、N-乙酰半乳糖胺和岩藻糖残基；黏液性腺泡细胞中半乳糖、N-乙酰半乳糖胺和N-乙酰葡糖胺残基也很丰富。
