Suleyman Demirel University, Faculty of Medicine, Department of Pharmacology, Medicine, Medical Device and Dermocosmetic Research and Application Laboratory-IDAL, 32260 Isparta, Turkey; YETEM, Innovative Technologies Research and Application Center, Suleyman Demirel University, 32260 Isparta, Turkey.
TUBITAK Marmara Research Center, Institute of Chemical Technology, 41470 Kocaeli, Turkey.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:1141-1152. doi: 10.1016/j.msec.2019.02.064. Epub 2019 Feb 18.
Hernia surgeries are at the top of the general surgery operations. However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem. One of the most preferred methods to prevent adhesion is the use of biomaterials. Polypropylene (PP) mesh is frequently preferred product in clinical applications owing to its mechanically robust structure against deformation within the body. However, PP meshes do not have anti-adhesive properties. Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use. ORC is not easily processable due to solubility limitations; and it must be used externally. In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure. We conducted in vitro cell culture studies to determine bio-compatibility performances. We evaluated the anti-adhesion and comprehensive bio-compatibility studies through in vivo experiments. The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.
疝修补术是普外科手术中最常见的手术之一。然而，内脏粘连是这些手术最严重的并发症之一，仍然是一个主要问题。预防粘连的最常用方法之一是使用生物材料。由于其在体内不易变形的机械坚固结构，聚丙烯（PP）网片在临床应用中经常被优先选用。然而，PP 网片没有抗粘连特性。另一方面，氧化再生纤维素（ORC）是临床应用中预防粘连最常用的产品之一。由于溶解度的限制，ORC 不易加工，必须外部使用。在这项研究中，我们首次设计了一种具有 ORC 的复合网片结构，并通过在 PP 网片上静电纺丝 ORC 微球和聚己内酯（PCL），生产出具有抗菌和抗粘连的双面复合网片。我们进行了体外细胞培养研究来确定生物相容性性能。我们通过体内实验评估了抗粘连和综合生物相容性研究。结果表明，ORC 的存在和通过 PCL 涂层优化 ORC 的降解在预防粘连方面起着重要作用，并引入了一种具有高效抗粘连性能的产品原型。
