MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China; School of Light Industry and Textile, Qiqihar University, Qiqihar 161000, China.
Carbohydr Polym. 2019 Mar 1;207:180-190. doi: 10.1016/j.carbpol.2018.10.077. Epub 2018 Oct 26.
Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed. A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions. In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite gauze was prepared. The N, O-CS/ORC composite gauze is degradable; in addition, the gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria. Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite gauze was confirmed in rabbit livers/ears as models. The results showed that the N, O-CS/ORC composite gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion. In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite gauze in preventing tissue adhesions after surgery. The results indicated that the N, O-CS/ORC composite gauze can significantly prevent postsurgical peritoneal adhesions. Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
组织粘连是手术后（尤其是腹部手术后）最常见的并发症之一，在受损组织愈合后会产生显著影响。在伤口部位和相邻组织之间放置物理屏障是一种方便且高效的技术，可以最大程度地减少或预防腹部粘连。在这项工作中，制备了 N，O-羧甲基壳聚糖/氧化再生纤维素（N，O-CS/ORC）复合纱布。N，O-CS/ORC 复合纱布可降解；此外，该纱布对金黄色葡萄球菌和大肠杆菌具有优异的抗菌功能。此外，在兔肝/耳模型中证实了 N，O-CS/ORC 复合纱布具有显著的止血功效。结果表明，N，O-CS/ORC 复合纱布对正常细胞无毒，能抑制成纤维细胞的粘连，表明其在预防组织粘连方面具有潜在的应用价值。此外，还使用大鼠腹部缺损-盲肠磨损模型来评估 N，O-CS/ORC 复合纱布在手术后预防组织粘连的功效。结果表明，N，O-CS/ORC 复合纱布能显著预防术后腹膜粘连。最后，研究了 N，O-CS/ORC 复合纱布的潜在抗粘连机制，这可能归因于 N，O-CS 和 ORC 的屏障功能和本能活性的结合。
