Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, PO Box: 14965-161, Tehran, Iran; Nanomaterials Group, Faculty of Engineering, Tarbiat Modares University, Tehran, PO Box: 14155-114, Tehran, Iran.
Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, PO Box: 14155-114, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:1485-1492. doi: 10.1016/j.msec.2019.02.063. Epub 2019 Feb 16.
Design of new multifunctional nanosystems to integrate diagnostic and therapeutic functions is a major area of interest in the field of nanobiotechnology and nanomedicine. In this study, application of a new magnetic multilayered nanobiohybrid system composed of magnetite nanoparticles, silica, and albumin as a theranostic agent was investigated. Once the clusters of magnetite nanoparticles were coated with a silica shell, bovine serum albumin was conjugated to the surface of activated amine-MS particles (BMS). Afterwards, a curcumin-loaded albumin shell was formed on BMS particles (CBBMS). The curcumin loading capacity and efficiency of the nanobiohybrid particles were 93 ± 6 (mg drug/g particles) and 24 ± 1.7 (wt%), respectively. A sustained release profile was observed for the curcumin in PBS which followed the first-order kinetic model. Cytotoxicity evaluation of CBBMS showed a striking reduced IC value (9.9 μM) in comparison to that of the free curcumin (16.2 μM), tested on SH-SY5Y cells using MTT assay. Finally, the in vitro relaxivity experiments indicated a high transverse relaxivity (r) of 369.7 mM s for the superparamagnetic nanobiohybrid particles in magnetic resonance imaging (MRI) tests. The obtained results in drug delivery and MRI tests recommend the as-prepared multilayered nanobiohybrid particles as an efficient and suitable system for theranostic applications.
设计集诊断和治疗功能于一体的新型多功能纳米系统是纳米生物技术和纳米医学领域的一个主要研究方向。在这项研究中，研究了由磁铁矿纳米颗粒、二氧化硅和白蛋白组成的新型磁性多层纳米生物杂交系统作为治疗药物的应用。一旦磁铁矿纳米颗粒簇被二氧化硅壳包裹，牛血清白蛋白就被连接到活化胺-MS 颗粒（BMS）的表面。之后，在 BMS 颗粒上形成负载姜黄素的白蛋白壳（CBBMS）。纳米生物杂交颗粒的姜黄素负载能力和效率分别为 93±6（mg 药物/g 颗粒）和 24±1.7（wt%）。在 PBS 中观察到姜黄素的缓释曲线，遵循一级动力学模型。通过 MTT 测定法在 SH-SY5Y 细胞上测试，CBBMS 的细胞毒性评估显示与游离姜黄素（16.2µM）相比，IC 值显著降低（9.9µM）。最后，体外弛豫率实验表明，在磁共振成像（MRI）测试中，超顺磁性纳米生物杂交颗粒的横向弛豫率（r）为 369.7mM·s。在药物输送和 MRI 测试中获得的结果表明，所制备的多层纳米生物杂交颗粒作为治疗药物应用的有效且合适的系统。
