Jeon Hamin, Pawlowski Michal E, Tkaczyk Tomasz S
Department of Bioengineering, Rice University, 6100 Main Street, Houston, TX 77005, USA.
Department of Electrical and Computer Engineering, Rice University, 6100 Main Street, Houston, TX 77005, USA.
Biomed Opt Express. 2019 Feb 25;10(3):1432-1445. doi: 10.1364/BOE.10.001432. eCollection 2019 Mar 1.
Fiber bundle endomicroscopy techniques have been used for numerous minimally invasive imaging applications. However, these techniques may provide limited spatial sampling due to the limited number of imaging cores inside the fiber bundle. Here, we present a custom-fabricated miniature objective that can be coupled to a fiber bundle and can overcome the fiber bundle's sampling threshold by utilizing the spectral encoding concept. The objective has an NA of 0.3 and an outer diameter of 2.4 mm, and can yield a maximum spatial resolution of 2 μm. The objective has been validated against a USAF resolution target and tissue samples, and as a result yielded images with higher resolution and more details after the spectral encoding concept was employed.
纤维束内镜技术已被用于众多微创成像应用中。然而,由于纤维束内成像核心数量有限,这些技术可能提供有限的空间采样。在此,我们展示了一种定制制造的微型物镜,它可以与纤维束耦合,并通过利用光谱编码概念克服纤维束的采样阈值。该物镜的数值孔径为0.3,外径为2.4毫米,可产生最大2微米的空间分辨率。该物镜已针对美国空军分辨率靶标和组织样本进行了验证,结果表明,采用光谱编码概念后,所产生的图像具有更高的分辨率和更多细节。