Shabtai Y, Gutnick D L
Appl Environ Microbiol. 1986 Jul;52(1):146-51. doi: 10.1128/aem.52.1.146-151.1986.
Mutants of Acinetobacter calcoaceticus RAG-1 that produced elevated levels of the polymeric bioemulsifier emulsan were isolated on the basis of their resistance to the cationic surfactant cetyltrimethylammonium bromide (CTAB). Such mutants showed maximum enhancement in both overall yield and specific productivity of some two- to threefold over that of the wild type. In addition, the effect was also observed in a resting cell system in the presence of chloramphenicol, indicating that the mutation is not simply the result of faster growth. When CTAB-tolerant mutants were subjected together with the sensitive parent to the detergent under growing conditions, only the mutants were found to grow. The results suggest that the mutation for CTAB resistance leads to enhanced capsule production. This was confirmed quantitatively by a specific enzyme-linked immunosorbent assay for the cell-bound emulsan minicapsule.
基于对阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）的抗性，分离出了乙酸钙不动杆菌RAG-1中产生聚合物生物乳化剂乳化素水平升高的突变体。这类突变体在总产量和比生产率方面比野生型均有最大约两到三倍的提高。此外，在存在氯霉素的静止细胞系统中也观察到了这种效应，这表明该突变并非仅仅是生长加快的结果。当在生长条件下将耐CTAB突变体与敏感亲本一起置于去污剂中时，仅发现突变体能够生长。结果表明，对CTAB的抗性突变导致荚膜产量增加。通过针对细胞结合的乳化素微胶囊的特异性酶联免疫吸附测定对这一点进行了定量确认。
