Deng Jun, Xia Zhiwei, Qiu Dongxu, Jiao Xiao, Xiao Bo, Zhou Wenbin, Yang Huan, Li Jing
Department of Neurology, Xiangya Hospital of Central South University, Changsha, Hunan, China.
Biomed Chromatogr. 2019 Jul;33(7):e4533. doi: 10.1002/bmc.4533. Epub 2019 Apr 12.
To investigate the characteristic of neuromyelitis optica spectrum disorder (NMOSD) in plasma and cerebrospinal fluid, UHPLC-MS was used to identify metabolites by metabolite extraction and on-machine detection. Multivariate analysis methods were used to complete differential metabolite screening and metabolic pathway analysis. The content of eight substances, such as tryptophan and l-glutamic acid in the plasma of NMOSD patients, was higher than that of the healthy control group. Moreover, no differential metabolite was found in the plasma of patients with AQP-4 antibody-positive and antibody-negative NMOSD. The content of five substances including 3-hydroxybutyric acid in cerebrospinal fluid of patients with NMOSD was reduced. We demonstrated that the distribution of metabolites in plasma between NMOSD patients and healthy counterparts was significantly different. However, there is no significant difference in plasma metabolites between AQP-4 antibody positive and negative NMOSD. There were some differences in metabolites between the cerebrospinal fluid of NMOSD patients and that of healthy controls. A variety of amino-acid abnormalities, sphingomyelin dysfunction, energy metabolism and mitochondrial dysfunction are involved in the pathogenesis of NMOSD.
为研究视神经脊髓炎谱系障碍（NMOSD）患者血浆和脑脊液的特征，采用超高效液相色谱-质谱联用技术（UHPLC-MS）通过代谢物提取和上机检测来鉴定代谢物。运用多变量分析方法完成差异代谢物筛选和代谢途径分析。NMOSD患者血浆中色氨酸、L-谷氨酸等8种物质的含量高于健康对照组。此外，AQP-4抗体阳性和抗体阴性的NMOSD患者血浆中未发现差异代谢物。NMOSD患者脑脊液中3-羟基丁酸等5种物质的含量降低。我们证明，NMOSD患者与健康对照者血浆中代谢物的分布存在显著差异。然而，AQP-4抗体阳性和阴性的NMOSD患者血浆代谢物无显著差异。NMOSD患者脑脊液与健康对照者脑脊液的代谢物存在一些差异。多种氨基酸异常、鞘磷脂功能障碍、能量代谢和线粒体功能障碍参与了NMOSD的发病机制。
