Kraljić Klara, Brkan Veronika, Škevin Dubravka, Srček Višnja Gaurina, Radošević Kristina
Laboratory for Oil and Fat Technology, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia.
Laboratory for Cell Technology, Application and Biotransformations, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia.
Lipids. 2019 Feb;54(2-3):189-200. doi: 10.1002/lipd.12132.
Rapeseed and its oil are the source of many biologically active substances. From crude rapeseed oil, canolol is isolated and from edible oil its dimer. Herein, we tested the biological activity of those two compounds isolated from the oils by determining their antioxidant capacity and in vitro cytotoxicity on human tumor cell lines. Canolol and its dimer showed antiproliferative activity on both cell lines with IC values of 46.45 μM in HeLa, and 51.19 μM in MCF7 cells, respectively. Evaluation of cell death was also done, while the oxygen radical absorbance capacity method confirmed that the canolol dimer has higher antioxidant potential than canolol. Stability of canolol and its dimer under different storage conditions showed that for a longer period of time both compounds should be stored in a freezer, but also that the dimer is more stable against degradation than canolol. Presented results indicate possible applications of canolol and its dimer in the food and pharmaceutical industry as a natural antioxidant and an anticancer agent, respectively.
油菜籽及其油类是多种生物活性物质的来源。从粗菜籽油中分离出菜油甾醇，从食用油中分离出其 dimer。在此，我们通过测定这两种从油类中分离出的化合物的抗氧化能力以及对人肿瘤细胞系的体外细胞毒性，来测试它们的生物活性。菜油甾醇及其 dimer 对两种细胞系均显示出抗增殖活性，在 HeLa 细胞系中的 IC 值分别为 46.45 μM，在 MCF7 细胞系中的 IC 值分别为 51.19 μM。同时还对细胞死亡情况进行了评估，而氧自由基吸收能力法证实菜油甾醇 dimer 比菜油甾醇具有更高的抗氧化潜力。菜油甾醇及其 dimer 在不同储存条件下的稳定性表明，这两种化合物在较长时间内都应保存在冰箱中，而且 dimer 比菜油甾醇更稳定，不易降解。所呈现的结果表明，菜油甾醇及其 dimer 分别作为天然抗氧化剂和抗癌剂在食品和制药行业可能具有应用价值。
