J Chin Med Assoc. 2019 May;82(5):413-418. doi: 10.1097/JCMA.0000000000000090.
The aim of this study was to investigate whether macrophages can participate in the immunosuppression of condyloma acuminatum (CA) by expressing PD-1/PD-L1.
The infiltration of macrophages and expression of programmed death-1 (PD-1) and PD-1 ligand 1 (PD-L1) on wart lesions and in normal skin tissues were detected by immunohistochemistry assay. The amounts of M1- and M2-type macrophages derived from THP-1 cells were measured by flow cytometry. The expression of cytokines on macrophages was examined by using enzyme-linked immunosorbent assay (ELISA). The protein expression of PD-1 and PD-L1 on macrophages was detected by western blotting.
The macrophages were significantly increased, while PD-1 and PD-L1 were highly expressed on wart lesions compared to normal controls. More M2-like macrophages than M1-type macrophages were present on wart lesions. The M2-like macrophages in the CA groups showed high expression of interleukin-10 (IL-10), transforming growth factor (TGF-β), PD-1, and PD-L1 compared to normal controls.
Macrophages participate in the immunosuppression of CA by expressing PD-1/PD-L1.
本研究旨在探讨巨噬细胞是否通过表达 PD-1/PD-L1 参与尖锐湿疣（CA）的免疫抑制。
采用免疫组织化学法检测疣状病变和正常皮肤组织中巨噬细胞的浸润及程序性死亡受体 1（PD-1）和 PD-1 配体 1（PD-L1）的表达。采用流式细胞术检测 THP-1 细胞来源的 M1 型和 M2 型巨噬细胞的数量。采用酶联免疫吸附试验（ELISA）检测巨噬细胞分泌的细胞因子。采用 Western blot 检测巨噬细胞 PD-1 和 PD-L1 的蛋白表达。
与正常对照组相比，疣状病变处巨噬细胞明显增多，PD-1 和 PD-L1 高表达。疣状病变处 M2 型巨噬细胞多于 M1 型巨噬细胞。与正常对照组相比，CA 组 M2 型巨噬细胞白细胞介素 10（IL-10）、转化生长因子（TGF-β）、PD-1 和 PD-L1 表达水平较高。
巨噬细胞通过表达 PD-1/PD-L1 参与 CA 的免疫抑制。
