Azuma H, Ishikawa M, Sekizaki S
Br J Pharmacol. 1986 Jun;88(2):411-5. doi: 10.1111/j.1476-5381.1986.tb10218.x.
In cascade perfusion and superfusion experiments on rabbit tissues, when acetylcholine (ACh) was introduced into the circuit so as to perfuse the aorta under perfusion with noradrenaline (NA), the effluent relaxed the transverse aortic strip which had been denuded of endothelium. The effluent from the perfused aorta which was capable of relaxing the transverse aortic strip also significantly inhibited platelet aggregation induced by arachidonic acid (AA) in a volume-related manner. The inhibitory activity was decreased by the prolongation of transit time before addition of the effluent to platelet-rich plasma. Neither the inhibition of AA-induced aggregation nor the relaxation of the transverse strip by the effluent could be observed after the removal of endothelium from the aorta, or after pretreatment of aorta with mepacrine or nordihydroguaiaretic acid (NDGA). The AA-induced platelet aggregation was unaffected by pretreatment of platelets with mepacrine or NDGA at the concentration tested. Pretreatment of aorta with indomethacin failed to modify the relaxation of the transverse strip induced by the effluent. These results strongly suggest that endothelium-derived vascular relaxant factor (EDRF) possesses inhibitory activity on AA-induced aggregation in addition to its vasodilator activity.
在对兔组织进行的级联灌注和超灌注实验中，当将乙酰胆碱（ACh）引入循环系统，以便在去甲肾上腺素（NA）灌注下灌注主动脉时，流出液使已去除内皮的主动脉横条松弛。能够使主动脉横条松弛的灌注主动脉流出液，也以与体积相关的方式显著抑制花生四烯酸（AA）诱导的血小板聚集。在将流出液加入富含血小板血浆之前，延长通过时间会降低抑制活性。在去除主动脉内皮后，或在用米帕林或去甲二氢愈创木酸（NDGA）预处理主动脉后，均未观察到流出液对AA诱导聚集的抑制作用以及对主动脉横条的松弛作用。在所测试的浓度下，用米帕林或NDGA预处理血小板，对AA诱导的血小板聚集没有影响。用吲哚美辛预处理主动脉，未能改变流出液诱导的主动脉横条松弛。这些结果有力地表明，内皮衍生的血管舒张因子（EDRF）除具有血管舒张活性外，还对AA诱导的聚集具有抑制活性。
