Biology Department, Trent University, 1600 West Bank Dr, Peterborough, ON K9J 7B8, Canada.
Viruses. 2019 Mar 19;11(3):276. doi: 10.3390/v11030276.
The characterization of the function of conserved viral genes is central to developing a greater understanding of important aspects of viral replication or pathogenesis. A comparative genomic analysis of the iridoviral genomes identified 26 core genes conserved across the family . Three of those conserved genes have no defined function; these include the homologs of (FV3) open reading frames (ORFs) 88R, 91R, and 94L. Conserved viral genes that have been previously identified are known to participate in a number of viral activities including: transcriptional regulation, DNA replication/repair/modification/processing, protein modification, and viral structural proteins. To begin to characterize the conserved FV3 ORFs 88R, 91R, and 94L, we cloned the genes and determined their intracellular localization. We demonstrated that 88R localizes to the cytoplasm of the cell while 91R localizes to the nucleus and 94L localizes to the endoplasmic reticulum (ER).
对保守病毒基因功能的描述是深入了解病毒复制或发病机制重要方面的核心。对虹彩病毒基因组的比较基因组分析确定了家族内 26 个保守的核心基因。其中三个保守基因没有明确的功能；这些包括（FV3）开放阅读框（ORF）88R、91R 和 94L 的同源物。以前确定的保守病毒基因已知参与多种病毒活动，包括：转录调控、DNA 复制/修复/修饰/加工、蛋白质修饰和病毒结构蛋白。为了开始描述保守的 FV3 ORFs 88R、91R 和 94L，我们克隆了这些基因并确定了它们的细胞内定位。我们证明 88R 定位于细胞质，91R 定位于细胞核，94L 定位于内质网（ER）。
