Shaoyong Weike, Li Qian, Ren Zhi-Qiang, Wei Chang-Sheng, Chu Gui-Yan, Dong Wu-Zi, Yang Gong-She, Pang Wei-Jun
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Theriogenology. 2019 May;130:146-156. doi: 10.1016/j.theriogenology.2019.03.005. Epub 2019 Mar 12.
ε-polylysine (ε-PL) has potent antibacterial effects and is often used in the food industry. However, no studies have clarified the antibacterial effects of ε-PL during storage of boar semen. In this study, boar semen samples were diluted with BTS buffer supplemented with different concentrations (0, 0.04, 0.08, 0.16, 0.32, 0.64, and 1.28 g/L) of ε-PL and different combinations of ε-PL plus gentamicin during liquid storage at 17 °C for 5 days. Bacterial concentrations, bacterial community compositions, sperm quality parameters, and in vitro fertilization (IVF) were evaluated in order to analyze the antibacterial effects of these parameters during boar semen preservation. The results indicated that the optimum concentration of ε-PL was 0.16 g/L, which significantly improved sperm quality parameters, including sperm motility, plasma membrane integrity, mitochondrial membrane potential, and acrosome integrity, and changed bacterial proliferation and composition (P < 0.05). Moreover, compared with the control group, IVF parameters in the treatment groups also significantly improved (P < 0.05), although there were no significant differences among treatment groups. Interestingly, the antibacterial effect of 0.16 g/L ε-PL in combination with 0.125 g/L gentamycin was similar to that of 0.25 g/L gentamicin alone. In conclusion, our results showed that 0.16 g/L ε-PL is promising for the replacement of gentamicin to improve sperm quality parameters, sperm capacitation, and IVF by reducing bacterial concentrations and disrupting bacterial community composition.
ε-聚赖氨酸（ε-PL）具有强大的抗菌作用，常用于食品工业。然而，尚无研究阐明ε-PL在公猪精液储存过程中的抗菌效果。在本研究中，将公猪精液样本用添加了不同浓度（0、0.04、0.08、0.16、0.32、0.64和1.28 g/L）ε-PL以及ε-PL与庆大霉素不同组合的BTS缓冲液进行稀释，于17°C下液态保存5天。评估细菌浓度、细菌群落组成、精子质量参数和体外受精（IVF），以分析这些参数在公猪精液保存过程中的抗菌效果。结果表明，ε-PL的最佳浓度为0.16 g/L，这显著改善了精子质量参数，包括精子活力、质膜完整性、线粒体膜电位和顶体完整性，并改变了细菌增殖和组成（P<0.05）。此外，与对照组相比，治疗组的IVF参数也显著改善（P<0.05），尽管各治疗组之间无显著差异。有趣的是，0.16 g/L ε-PL与0.125 g/L庆大霉素联合使用的抗菌效果与单独使用0.25 g/L庆大霉素相似。总之，我们 的结果表明，0.16 g/L ε-PL有望替代庆大霉素，通过降低细菌浓度和破坏细菌群落组成来改善精子质量参数、精子获能和IVF。
