细菌与公猪精液储存：进展与挑战
Bacteria and Boar Semen Storage: Progress and Challenges.
作者信息
Contreras María José, Núñez-Montero Kattia, Bruna Pablo, García Matías, Leal Karla, Barrientos Leticia, Weber Helga
机构信息
Extreme Environments Biotechnology Lab, Center of Excellence in Translational Medicine, Universidad de La Frontera, Av. Alemania 0458, Temuco 01145, Chile.
Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, Avenida Francisco Salazar, Temuco 01145, Chile.
出版信息
Antibiotics (Basel). 2022 Dec 10;11(12):1796. doi: 10.3390/antibiotics11121796.
Abstract
Porcine breeding today is based on artificial insemination with chilled semen. This is stored at 5 °C with antibiotic supplementation to avoid bacteriospermia. There are many negative consequences on sperm quality and functionality as a result of bacterial contamination, as well as on the health of the sow. Nowadays, various techniques are being developed to reduce the indiscriminate use of antibiotics and thus avoid the generation of antibiotic resistance genes. This review aims to inform about the bacterial contamination consequences of storing liquid semen from boar and to provide an update on current methods and alternatives to antibiotic use in cold storage.
摘要
如今，猪的繁殖基于使用冷藏精液的人工授精技术。精液保存在5°C，并添加抗生素以避免细菌感染。细菌污染会对精子质量和功能以及母猪健康产生许多负面影响。如今，人们正在开发各种技术以减少抗生素的滥用，从而避免产生抗生素抗性基因。这篇综述旨在介绍公猪精液冷藏时细菌污染的后果，并提供关于当前冷藏中抗生素使用的方法及替代方案的最新信息。
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2
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3
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Animals (Basel). 2021 Nov 20;11(11):3320. doi: 10.3390/ani11113320.
4
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Antibiotics (Basel). 2021 Oct 29;10(11):1319. doi: 10.3390/antibiotics10111319.
5
Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine.
Molecules. 2021 Oct 13;26(20):6183. doi: 10.3390/molecules26206183.
6
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7
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Biomed Res Int. 2021 Jun 2;2021:5573237. doi: 10.1155/2021/5573237. eCollection 2021.
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Reprod Domest Anim. 2021 Sep;56(9):1200-1208. doi: 10.1111/rda.13975. Epub 2021 Jun 17.
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