利用向日葵根际促生菌菌株（Paenibacillus sp. IITISM08、Bacillus sp. PRB77 和 Bacillus sp. PRB101）对污染土壤中硫丹的降解作用。
Influence of plant growth promoting rhizobacterial strains Paenibacillus sp. IITISM08, Bacillus sp. PRB77 and Bacillus sp. PRB101 using Helianthus annuus on degradation of endosulfan from contaminated soil.
机构信息
Laboratory of Applied Microbiology, Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, Dhanbad, 826 004, Jharkhand, India.
Laboratory of Applied Microbiology, Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, Dhanbad, 826 004, Jharkhand, India.
出版信息
Chemosphere. 2019 Jun;225:479-489. doi: 10.1016/j.chemosphere.2019.03.037. Epub 2019 Mar 9.
Endosulfan is a broad spectrum insecticide used in agriculture for protection of various food and non-food crops. It is persistent in nature and hence found in soil, air and water. The potential use of plants and microorganisms for the removal of endosulfan from soil was studied. Helianthus annuus plant was grown in soil spiked with 5, 10, 25 and 50 mg kg concentrations of endosulfan and inoculated with plant growth promoting rhizobacterial strains Paenibacillus sp. IITISM08, Bacillus sp. PRB77 and Bacillus sp. PRB101 for 40, 80 and 120 days. Potential of plant for endosulfan uptake was evaluated by investigating the endosulfan levels in plant tissues (root and shoot). The results indicated that endosulfan accumulation followed the pattern of root > shoot as well as decrease in uptake of endosulfan in root and shoot of a plant grown in bacterial inoculated soil as compared to un-inoculated soil. Bacterial inoculation had a positive effect on endosulfan degradation. Maximum degradation of 92% at 5 mg kg of endosulfan in soil was observed on inoculation with PRB101 after 120 days of inoculation. The results showed that plant growth promoting bacteria enhances plant biomass production. Lipid peroxidation was also estimated by determining the malondialdehyde (MDA) production, which is a biomarker of oxidative damage. Decrease in MDA formation by root and leaves of plants grown in the bacteria inoculated plant was also observed. The results suggested the effectiveness of plant growth promoting rhizobacteria to boost accumulation potential, biomass production and enhance remediation of endosulfan contaminated soil.
硫丹是一种广谱杀虫剂，用于保护各种粮食和非粮食作物。它在自然界中具有持久性，因此存在于土壤、空气和水中。本研究探讨了植物和微生物从土壤中去除硫丹的潜力。在土壤中添加 5、10、25 和 50mg/kg 的硫丹后，种植向日葵，并接种植物促生根际细菌菌株 Paenibacillus sp. IITISM08、芽孢杆菌 PRB77 和芽孢杆菌 PRB101，培养 40、80 和 120 天。通过研究植物组织（根和茎）中的硫丹水平来评估植物对硫丹的吸收潜力。结果表明，硫丹的积累模式为根>茎，与未接种土壤相比，接种细菌的土壤中植物根和茎对硫丹的吸收减少。细菌接种对硫丹降解有积极影响。在接种 PRB101 120 天后，土壤中 5mg/kg 硫丹的最大降解率为 92%。结果表明，植物促生细菌可提高植物生物量的产生。还通过测定丙二醛（MDA）的产生来估计脂质过氧化，MDA 是氧化损伤的生物标志物。在接种细菌的植物中，根和叶中 MDA 的形成减少。结果表明，植物促生根际细菌可有效提高积累潜力、生物量的产生，并增强对受硫丹污染土壤的修复。
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