Laboratory of Applied Microbiology Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, 826 004, Jharkhand, India.
Laboratory of Applied Microbiology Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, 826 004, Jharkhand, India.
Chemosphere. 2021 Sep;279:130589. doi: 10.1016/j.chemosphere.2021.130589. Epub 2021 Apr 16.
This study aimed to assess the role of Solanum lycopersicum and plant growth promoting rhizobacterial (PGPR) strains to remove endosulfan present in the soil. S. lycopersicum was grown in endosulfan amended soil (5, 10, 25, and 50 mg kg) inoculated with PGPR strains for 40, 80, and 120 days. The influence of PGPR inoculation on endosulfan accumulation in plant tissues, endosulfan degradation in soil, and plant growth parameters were evaluated. The oxidative stress tolerance was assessed by determining the malondialdehyde formation in S. lycopersicum planted in endosulfan stressed soil inoculated with PGPR strains. The results showed that uptake of endosulfan followed root > shoot pathway in association with a reduction in endosulfan accumulation in inoculated plants as related to un-inoculated plants. Moreover, inoculation of PGPR strains showed a beneficial influence on the degradation of endosulfan, Bacillus sp. PRB101 showed maximum degradation (89% at 5 mg kg of soil) of endosulfan at 120 days after sowing. Furthermore, the content of malondialdehyde was lower in inoculated plants as related to un-inoculated plants. Inoculation of PGPR strains efficiently enhanced plant biomass. The findings showed the effectiveness of PGPR strains to increase the decontamination of endosulfan stressed soil and decline endosulfan concentration in the plant tissues.
本研究旨在评估番茄和植物促生根际细菌（PGPR）菌株在去除土壤中存在的硫丹的作用。将番茄种植在添加了硫丹的土壤中（5、10、25 和 50 mg kg），并用 PGPR 菌株接种 40、80 和 120 天。评估了 PGPR 接种对植物组织中硫丹积累、土壤中硫丹降解和植物生长参数的影响。通过测定种植在添加了硫丹的土壤中并接种了 PGPR 菌株的番茄中的丙二醛形成来评估氧化应激耐受性。结果表明，硫丹的吸收遵循根>茎途径，与未接种植物相比，接种植物中的硫丹积累减少。此外，PGPR 菌株的接种对硫丹的降解表现出有益的影响，芽孢杆菌 PRB101 在播种后 120 天时对硫丹的最大降解率（在 5 mg kg 土壤中为 89%）。此外，接种植物中的丙二醛含量低于未接种植物。PGPR 菌株的接种有效地提高了植物生物量。研究结果表明，PGPR 菌株可有效增强受硫丹污染土壤的净化能力，并降低植物组织中的硫丹浓度。
