Yang Bo, Thomas Andrew L, Greenlief C Michael
Department of Chemistry, University of Missouri, Columbia, MO 65211, USA.
Division of Plant Sciences, Southwest Research Center, University of Missouri, Mt. Vernon, MO 65712, USA.
Proteomes. 2019 Mar 20;7(1):10. doi: 10.3390/proteomes7010010.
American elderberry ( subsp. ) is a rapidly growing specialty crop in Missouri and eastern North America. Nitrogen (N) is a major nutrient involved in plant growth and development. However, proteome changes for different genotypes of elder in response to varying levels of N-treatment remain undefined. To reveal plant responses to N, comparative proteomic analyses were performed to determine consistent changes in three genotypes of elderberry leaves (Adams II, Bob Gordon and Wyldewood) grown under different N-fertilizer treatments. 165 proteins separated by two dimensional gel electrophoresis showed significant differences in abundance ( < 0.05 and greater than 2-fold). Principal component analysis of the abundance profiles of these proteins revealed Bob Gordon as a distinct genotype. The 165 proteins were identified by mass spectrometry and showed similar functional distributions in these genotypes underlying the N-treatment. Among the proteins identified, 23 are mainly involved in photosynthesis, protein metabolism and redox homeostasis. Their abundance profiles were not altered upon exposure to N or genotype. These results provide novel insights into plant responses to fertilizer treatment at the proteome level and could lead to a better understanding of molecular mechanisms of elderberry growth.
美洲接骨木（亚种）是密苏里州和北美东部一种快速发展的特色作物。氮（N）是参与植物生长发育的主要养分。然而，不同接骨木基因型对不同水平氮处理的蛋白质组变化仍不明确。为揭示植物对氮的反应，进行了比较蛋白质组学分析，以确定在不同氮肥处理下生长的三种接骨木叶片基因型（亚当斯二号、鲍勃·戈登和怀尔德伍德）中的一致性变化。通过二维凝胶电泳分离出的165种蛋白质在丰度上显示出显著差异（P<0.05且大于2倍）。对这些蛋白质丰度谱的主成分分析表明鲍勃·戈登是一个独特的基因型。通过质谱鉴定了这165种蛋白质，并且在氮处理下这些基因型中显示出相似的功能分布。在鉴定出的蛋白质中，23种主要参与光合作用、蛋白质代谢和氧化还原稳态。它们的丰度谱在暴露于氮或基因型时没有改变。这些结果在蛋白质组水平上为植物对肥料处理的反应提供了新的见解，并有助于更好地理解接骨木生长的分子机制。
