Nursing Department and Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Xi'an Medical College, Xi'an, P. R. China.
Department of Obstetrics and Gynecology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, P. R. China.
J Matern Fetal Neonatal Med. 2020 Oct;33(20):3399-3408. doi: 10.1080/14767058.2019.1572741. Epub 2019 Mar 21.
To examine the cellular distribution and the expression of CD200 and its receptor 1 (CD200R1) in human deciduas in first-trimester pregnant women with spontaneous early abortion (SEA) and normal pregnancy, and to explore their role in the etiology of SEA. Thirty-five women at 6-10-week gestation with SA and 30 women of similar gestational age with a healthy pregnancy were recruited. Expression of CD200 and CD200R1 in the deciduas was determined using immunohistochemistry, confocal laser scanning microscope, Western blot, and real-time PCR (RT-PCR). The decidual stromal cells, glandular epithelial cells, and vessel endothelial cells during the first trimester of pregnancy express both CD200 and CD200R1 proteins. During this period, the expression of CD200 in glandular epithelial cells and vessel endothelial cells is significantly higher in normal pregnancy than that in women with SEA (0.3079 ± 0.0674 versus 0.2735 ± 0.0515; 0.4077 ± 0.1366 versus 0.3249 ± 0.0993); the expression of CD200R1 in stromal cells, decidual stromal cells, glandular epithelial cells is significantly higher during normal pregnancy than SEA (0.2574 ± 0.0588 versus 0.2292 ± 0.0415; 0.3617 ± 0.1046 versus 0.2804 ± 0.0640). Western blot analysis showed an approximately 44% decrease in CD200R1expression in decidua in the SEA versus the controls. Finally, in decidua, the expression of both CD200 protein and CD200R1 transcript are significantly higher in healthy first-trimester pregnancy than in SEA (CD200: 2.2089 ± 1.2754 versus 0.7241 ± 0.2143; CD200R1: 15.7843 ± 10.7085 versus 7.3381 ± 5.8529). Women with SEA have a lower level of CD200 and CD200R1 expression in deciduas compared with normal pregnant women suggesting that under physiological conditions, CD200 and CD200R1 expression by deciduas is important to prevent fetal loss ensure a successful pregnancy.
为了研究 CD200 和其受体 1（CD200R1）在自然流产（SEA）和正常妊娠早期妊娠蜕膜中的细胞分布和表达，并探讨它们在 SEA 病因学中的作用，招募了 35 名 6-10 周妊娠伴 SEA 和 30 名具有相似妊娠年龄的正常妊娠妇女。使用免疫组织化学，共聚焦激光扫描显微镜，Western blot 和实时 PCR（RT-PCR）确定蜕膜中 CD200 和 CD200R1 的表达。在妊娠的第一 trimester 中，蜕膜基质细胞，腺上皮细胞和血管内皮细胞均表达 CD200 和 CD200R1 蛋白。在此期间，正常妊娠中腺上皮细胞和血管内皮细胞中 CD200 的表达明显高于 SEA（0.3079±0.0674 对 0.2735±0.0515；0.4077±0.1366 对 0.3249±0.0993）；正常妊娠中基质细胞，蜕膜基质细胞，腺上皮细胞中 CD200R1 的表达明显高于 SEA（0.2574±0.0588 对 0.2292±0.0415；0.3617±0.1046 对 0.2804±0.0640）。Western blot 分析表明，SEA 中 CD200R1 的表达比对照组约降低 44％。最后，在蜕膜中，正常妊娠的第一 trimester 中 CD200 蛋白和 CD200R1 转录本的表达均明显高于 SEA（CD200：2.2089±1.2754 对 0.7241±0.2143；CD200R1：15.7843±10.7085 对 7.3381±5.8529）。与正常妊娠妇女相比，SEA 患者的蜕膜中 CD200 和 CD200R1 的表达水平较低，这表明在生理条件下，蜕膜中 CD200 和 CD200R1 的表达对于防止胎儿丢失和确保成功妊娠很重要。
