Department of Analytical Chemistry, University of Granada, C/Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. Del Conocimiento s/n., Edificio BioRegion, 18016 Granada, Spain.
Institute of Molecular and Cell Biology (IBMC), Miguel Hernández University, Avda. de la Universidad, s/n, 03202 Elche, Spain; CIBER, Fisiopatología de la Obesidad y la Nutrición, CIBERobn (CB12/03/30038), Carlos III Health Institute (ISCIII), 07122 Palma de Mallorca, Spain.
Food Res Int. 2019 Apr;118:96-100. doi: 10.1016/j.foodres.2018.02.027. Epub 2018 Feb 13.
Energy metabolism is one of the main sources of reactive oxygen species leading to oxidation and inflammation in pathophysiological processes. Lipopolysaccharide (LPS)-activated mouse embryonic fibroblast (MEF) cell lines from knock-out mice for paraoxonase-1 and from transgenic mice overexpressing monocyte chemoattractant protein-1 were obtained as model of pro-oxidant and pro-inflammatory scenarios. Theobroma cacao and Lippia citriodora (worldwide consumed and common ingredient of many food products) were tested in these cell models to assess the action of polyphenols in the energy management. Our metabolomics experiments show a different behavior of polyphenols: T. cacao extract partially reverts the effect of LPS in a pro-oxidant scenario through the antioxidant properties of theobromine, flavonols and procyanidins, while L. citriodora seems to act mainly in a pro-inflammatory cell model through the action of verbascoside decreasing the production of pro-inflammatory cytokines and MCP-1. Nevertheless, the action of polyphenols cannot be attributed only to a mechanism of action but the sum of different modulations in biological pathways. The capacity of both plant extracts to decrease α-ketoglutarate levels merits special attention due to the implications in future medicine. The action of polyphenols modulating oxidative stress, cytokine production and epigenetic changes make an interesting source of bioactive compounds for nutraceutical or functional food purposes.
能量代谢是导致病理生理过程中氧化和炎症的活性氧物种的主要来源之一。我们获得了过氧化物酶 1 基因敲除小鼠和单核细胞趋化蛋白 1 转基因小鼠的脂多糖（LPS）激活的胚胎成纤维细胞（MEF）细胞系，作为促氧化剂和促炎情况的模型。可可和香蜂草（在世界范围内食用，是许多食品的常见成分）在这些细胞模型中进行了测试，以评估多酚在能量管理中的作用。我们的代谢组学实验表明多酚具有不同的作用：可可提取物通过可可因、类黄酮和原花青素的抗氧化特性，部分逆转了 LPS 在促氧化剂情况下的作用，而香蜂草似乎主要通过减少促炎细胞因子和 MCP-1 产生的圣草次甙作用于促炎细胞模型。然而，多酚的作用不能仅仅归因于一种作用机制，而是生物途径中不同调节的总和。由于多酚在未来医学中的应用潜力，两种植物提取物降低α-酮戊二酸水平的能力值得特别关注。多酚调节氧化应激、细胞因子产生和表观遗传变化的作用，为营养保健品或功能性食品的生物活性化合物提供了一个有趣的来源。
