重组DNA衍生的人及小鼠γ干扰素对小鼠肝脏药物代谢的影响。

The influence of recombinant DNA-derived human and murine gamma interferons on mouse hepatic drug metabolism.

作者信息

Franklin M R, Finkle B S

出版信息

Fundam Appl Toxicol. 1986 Jul;7(1):165-9. doi: 10.1016/0272-0590(86)90210-1.

Abstract

Human gamma interferon given for up to 5 days by subcutaneous infusion or intraperitoneal injection did not significantly alter mouse hepatic microsomal oxidative drug-metabolizing enzyme activities. In contrast, murine gamma interferon and human alpha interferon given for 5 days at the same dose (10(7) units/kg) caused 25 and 50% decreases, respectively, in hepatic microsomal cytochrome P-450 concentrations. The human alpha interferon-induced decline in cytochrome P-450 was accompanied by a significant drop in p-nitroanisole demethylase activity and significant elevations in serum alanine aminotransferase and cytosolic glutathione S-transferase activities. An elevation in glutathione-S-transferase was the only significant change found following human gamma interferon administration. Microsomal UDP-glucuronosyltransferase activity was unaffected by any interferon.

摘要

通过皮下输注或腹腔注射给予长达5天的人γ干扰素，并未显著改变小鼠肝脏微粒体氧化药物代谢酶的活性。相比之下，以相同剂量（10⁷单位/千克）给予5天的鼠γ干扰素和人α干扰素，分别导致肝脏微粒体细胞色素P - 450浓度降低25%和50%。人α干扰素诱导的细胞色素P - 450下降伴随着对硝基苯甲醚脱甲基酶活性的显著下降以及血清丙氨酸转氨酶和胞质谷胱甘肽S - 转移酶活性的显著升高。给予人γ干扰素后，唯一显著的变化是谷胱甘肽 - S - 转移酶升高。微粒体UDP - 葡萄糖醛酸基转移酶活性不受任何干扰素的影响。