Program in Primary Immunodeficiency and Aging, Division of Basic and Clinical Immunology, Jeffrey Modell Diagnostic Center for Primary Immunodeficiencies, University of California, Irvine, Irvine, CA, United States.
Front Immunol. 2019 Mar 7;10:399. doi: 10.3389/fimmu.2019.00399. eCollection 2019.
CMV colitis has been reported in immunocompromized patients with severe deficiency of CD4+ T cells and T cell functions. In this study we present an extensive immunological analysis in a patient with primary hypogammaglobulinemia and CMV colitis who had normal numbers of CD3+T, CD4+T and CD8+T cells, and normal T cell proliferative responses to mitogens and recall antigens. Naïve (T), central (T), and effector (T) memory subsets of CD4+ and CD8+ T cells, Granzyme+ and Perforin+ CD8+ T cells, PD-1+ T cells, CD4 Treg, CD8 Treg, and CMV tetramer specific CD8+ T cells were analyzed with specific antibodies and isotype controls using multicolor flow cytometry. CD8 T, Granzyme+ and Perforin+, and PD-1 CD8+T cells were increased, whereas CD8 T and CD8 T cells were decreased in the patient as compared to controls. CMV tetramer+ CD8+ T cells were decreased in the patient. These data demonstrate that a deficiency of CMV-specific CD8+ T cells even in the presence of normal CD4+ T cell numbers and normal T cell functions may predispose patients with primary hypogammaglobulinemia to CMV colitis.
CMV 结肠炎已在严重缺乏 CD4+T 细胞和 T 细胞功能的免疫功能低下患者中报道。在这项研究中，我们对一名患有原发性低丙种球蛋白血症和 CMV 结肠炎的患者进行了广泛的免疫学分析，该患者的 CD3+T、CD4+T 和 CD8+T 细胞数量正常，对有丝分裂原和回忆抗原的 T 细胞增殖反应正常。使用多色流式细胞术，用特异性抗体和同型对照分析了 CD4+和 CD8+T 细胞的幼稚（T）、中央（T）和效应（T）记忆亚群、Granzyme+和 Perforin+CD8+T 细胞、PD-1+T 细胞、CD4 Treg、CD8 Treg 和 CMV 四聚体特异性 CD8+T 细胞。与对照相比，患者的 CD8+T、Granzyme+和 Perforin+和 PD-1+CD8+T 细胞增加，而 CD8+T 和 CD8+T 细胞减少。患者的 CMV 四聚体+CD8+T 细胞减少。这些数据表明，即使在 CD4+T 细胞数量正常和 T 细胞功能正常的情况下，CMV 特异性 CD8+T 细胞的缺乏也可能使原发性低丙种球蛋白血症患者易患 CMV 结肠炎。
