色氨酸羟化酶在有症状和无症状感染的胃黏膜中的表达

Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic infection.

作者信息

Chojnacki Cezary, Popławski Tomasz, Błońska Aleksandra, Błasiak Janusz, Romanowski Marek, Chojnacki Jan

机构信息

Department of Clinical Nutrition and Gastroenterological Diagnostics, Medical University of Lodz, Lodz, Poland.

Department of Molecular Genetics, University of Lodz, Lodz, Poland.

出版信息

Arch Med Sci. 2019 Mar;15(2):416-423. doi: 10.5114/aoms.2018.76928. Epub 2018 Jul 5.

INTRODUCTION

infection induces clinical symptoms in 15-20% of subjects, and the reason for this variation is still not clear. The aim of the present study is to evaluate the expression of tryptophan hydroxylase (TpH-1) in gastric mucosa of patients with symptomatic and asymptomatic infection in relation to the intensity of bacterial colonization and severity of dyspeptic symptoms.

MATERIAL AND METHODS

Ninety subjects (aged 35-49 years) were enrolled in the study and separated into 3 groups of 30 subjects each. Group I - healthy volunteers without infection, group II - subjects with asymptomatic infection, group III - -infected patients with dyspeptic symptoms, mainly fasting and nocturnal epigastric pain. To diagnose infection the urea breath test (UBT-13C) and histological analysis were performed. The level of mRNA expression of tryptophan hydroxylase (TpH-1) was estimated in gastric mucosa with RT-PCR.

RESULTS

The expression of this enzyme in antral mucosa was 2.69 ±0.97 in group I, 2.28 ±0.69 in group II ( > 0.05) and 4.40 ±1.64 in group III ( < 0.001). The levels of expression of TpH-1 in gastric body mucosa were 2.16 ±0.70, 1.57 ±0.52 ( > 0.05) and 3.40 ±1.51 ( < 0.001), respectively. In group III a positive correlation was found between intensity of colonization and TpH-1 expression as well as between TpH-1 expression and severity of dyspeptic symptoms.

CONCLUSIONS

Increased expression of TpH-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia.

引言

感染在15%至20%的受试者中会引发临床症状，而这种差异的原因尚不清楚。本研究的目的是评估有症状和无症状感染患者胃黏膜中色氨酸羟化酶（TpH-1）的表达情况，并将其与细菌定植强度和消化不良症状的严重程度相关联。

材料与方法

90名年龄在35至49岁之间的受试者参与了本研究，并被分为3组，每组30人。第一组——无感染的健康志愿者，第二组——无症状感染的受试者，第三组——有消化不良症状（主要是空腹和夜间上腹部疼痛）的感染患者。通过尿素呼气试验（UBT-13C）和组织学分析来诊断感染。采用逆转录聚合酶链反应（RT-PCR）估计胃黏膜中色氨酸羟化酶（TpH-1）的mRNA表达水平。

结果

该酶在胃窦黏膜中的表达在第一组为2.69±0.97，第二组为2.28±0.69（P>0.05），第三组为4.40±1.64（P<0.001）。TpH-1在胃体黏膜中的表达水平分别为2.16±0.70、1.57±0.52（P>0.05）和3.40±1.51（P<0.001）。在第三组中，发现细菌定植强度与TpH-1表达之间以及TpH-1表达与消化不良症状的严重程度之间存在正相关。