Department of Chemistry , Indian Institute of Technology Delhi , Hauz Khas, New Delhi 110016 , India.
J Phys Chem B. 2019 Apr 11;123(14):3112-3117. doi: 10.1021/acs.jpcb.9b01676. Epub 2019 Apr 3.
We report herewith tryptophan (Trp)-conjugated peptidomimetics that show intramolecular through-space association between the Trp units. Our investigation revealed that the proximal placement of Trp can lead to the emergence of a new and unanticipated fluorescent entity constituting a Trp-Trp dimer. Proton-induced modulation of fluorescence is a consequence of this work. Investigations with control compounds unequivocally revealed that the fluorescence property is not originated from the localized excited state but from the unprecedented Trp-Trp intramolecular dimer in the ground state itself. The present findings will initiate the biophysical scientists to have a relook at the fluorescence properties of Trp-containing proteins.
我们在此报告色氨酸（Trp）缀合的肽模拟物，这些模拟物显示色氨酸单元之间的分子内空间缔合。我们的研究表明，色氨酸的近端放置可以导致新的和意外的荧光实体的出现，构成色氨酸-色氨酸二聚体。这项工作导致了质子诱导的荧光调制。用对照化合物进行的研究明确揭示，荧光性质不是源自局部激发态，而是源自前所未有的色氨酸-色氨酸分子内二聚体在基态本身。目前的发现将促使生物物理科学家重新审视含色氨酸蛋白质的荧光性质。
