Blue M L, Daley J F, Levine H, Craig K A, Schlossman S F
J Immunol. 1986 Aug 15;137(4):1202-7.
Biosynthetic labeling with 35S-methionine and 35S-cysteine of isolated T4+ cells from Con A-activated T cells demonstrated that the T8 antigen was synthesized by activated T4+ cells. Two-color fluorescence analysis of the activated T cell population from which the T4+ fraction was obtained showed that both T4+T8- and T4+T8+ cells were present. The T8 antigen that was immunoprecipitated by monoclonal anti-T8 from activated T4+ cells migrated with an electrophoretic mobility corresponding to an m.w. of approximately 33,000, a previously reported m.w. value for T8 antigen. Con A activation of highly purified peripheral T4+T8- and T8+T4- subsets indicated that both T4+T8- and T8+T4- cells can give rise to T4+T8+ cells. However, substantial T4, T8 coexpression by T4+T8- cells required a signal from T8+T4- cells which could be supplied by incubating T4+T8- cells with irradiated T8+ cells or the supernatant from Con A-activated T8+T4- cells. The generation of T4+T8+ cells from a subset of T4+T8- T cells may be an important mechanism in immune activation and/or the further differentiation of peripheral T4+ cells.
用³⁵S-甲硫氨酸和³⁵S-半胱氨酸对从刀豆蛋白A激活的T细胞中分离出的T4⁺细胞进行生物合成标记，结果表明T8抗原是由激活的T4⁺细胞合成的。对获得T4⁺亚群的激活T细胞群体进行双色荧光分析显示，同时存在T4⁺T8⁻和T4⁺T8⁺细胞。从激活的T4⁺细胞中用单克隆抗T8免疫沉淀的T8抗原，其电泳迁移率对应的分子量约为33,000，这是先前报道的T8抗原的分子量值。对高度纯化的外周T4⁺T8⁻和T8⁺T4⁻亚群进行刀豆蛋白A激活，结果表明T4⁺T8⁻和T8⁺T4⁻细胞都可以产生T4⁺T8⁺细胞。然而，T4⁺T8⁻细胞大量共表达T4、T8需要来自T8⁺T4⁻细胞的信号，该信号可以通过将T4⁺T8⁻细胞与经辐照的T8⁺细胞或刀豆蛋白A激活的T8⁺T4⁻细胞的上清液孵育来提供。从T4⁺T8⁻T细胞亚群产生T4⁺T8⁺细胞可能是免疫激活和/或外周T4⁺细胞进一步分化的重要机制。
